SARS- and Other Coronaviruses : Laboratory Protocols - Cavanagh, Dave.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2008)
Detection and Discovery of Coronaviruses -- A Pancoronavirus RT-PCR Assay for Detection of All Known Coronaviruses -- Detection of Group 1 Coronaviruses in Bats Using Universal Coronavirus Reverse Transcription Polymerase Chain Reactions -- Detection and Sequence Characterization of the 3?-End of Coronavirus Genomes Harboring the Highly Conserved RNA Motif s2m -- RT-PCR Detection of Avian Coronaviruses of Galliform Birds (Chicken, Turkey, Pheasant) and in a Parrot -- Detection of Group 2a Coronaviruses with Emphasis on Bovine and Wild Ruminant Strains -- Detection of SARS Coronavirus in Humans and Animals by Conventional and Quantitative (Real Time) Reverse Transcription Polymerase Chain Reactions -- Detection of New Viruses by VIDISCA -- Isolation, Growth, Titration, and Purification of Coronaviruses -- Titration of Human Coronaviruses, HCoV-229E and HCoV-OC43, by an Indirect Immunoperoxidase Assay -- The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration -- Isolation and Propagation of Coronaviruses inEmbryonated Eggs -- Large-Scale Preparation of UV-Inactivated SARS Coronavirus Virions for Vaccine Antigen -- Structure of Covs Analyzed by Electron Microscopy -- Purification and Electron Cryomicroscopy of Coronavirus Particles -- Expression of Coronavirus Proteins and Crystallization -- Production of Coronavirus Nonstructural Proteins in Soluble Form for Crystallization -- Raising Antibodies to Coronavirus Proteins -- Generating Antibodies to the Gene 3 Proteins of Infectious Bronchitis Virus -- Establishment and Characterization of Monoclonal Antibodies Against SARS Coronavirus -- Production of Monospecific Rabbit Antisera Recognizing Nidovirus Proteins -- Manipulating the Genomes of Coronaviruses -- Manipulation of the Coronavirus Genome Using Targeted RNA Recombination with Interspecies Chimeric Coronaviruses -- Generation of Recombinant Coronaviruses Using Vaccinia Virus as the Cloning Vector and Stable Cell Lines Containing Coronaviral Replicon RNAs -- Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses -- Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes -- Systematic Assembly and Genetic Manipulation of the Mouse Hepatitis Virus A59 Genome -- Identifying Coronavirus Receptors -- Identification of Sugar Residues Involved in the Binding of TGEV to Porcine Brush Border Membranes -- Pseudotyped Vesicular Stomatitis Virus for Analysis of Virus Entry Mediated by SARS Coronavirus Spike Proteins.
Keywords: Medical microbiology; Infectious diseases; Virology; Laboratory medicine; Medical Microbiology; Infectious Diseases; Virology; Laboratory Medicine