Snake and Spider Toxins : Methods and Protocols - Priel, Avi.; SpringerLink (Online service) (New York, NY : Springer US : Imprint: Humana, 2020)
Snake- and Spider-Venom-Derived Toxins as Lead Compounds for Drug Development -- Analytics for Bioactivity Profiling of Complex Mixtures with a Focus on Venoms -- Venom Collection from Spiders and Snakes: Voluntary and Involuntary Extractions ("Milking") and Venom Gland Extractions -- Production and Purification of Recombinant Toxins -- RNA-Sequencing of Snake Venom Glands -- Exploring Toxin Evolution: Venom Protein Transcript Sequencing and Transcriptome-Guided High-Throughput Proteomics -- Three-Dimensional Structure Determination of Peptides using Solution Nuclear Magnetic Resonance Spectroscopy -- Determining the Structures of the Snake and Spider Toxins by X-Rays -- MALDI-TOF Mass Spectrometric Profiling of Spider Venoms -- Methods for Evaluation of a Snake Venom Derived Disintegrin in Animal Models of Human Cancer -- Cell-Based Adhesion Assays for Isolation of Snake Venom's Integrin Antagonists -- Using C. elegans to Study the Effects of Toxins in Sensory Ion Channels In Vivo -- Measurements of Cell Death Induced by Snake and Spider's Venoms and Derived Toxins -- Using Toxins in Brain Slice Recordings -- High-Throughput Calcium Imaging Screen of Toxins' Function in Dissociated Sensory Neurons -- Synthesizing and Expressing Native Ion Channels.
Keywords: Pharmacology; Pharmacology/Toxicology.