Synthetic Metabolic Pathways : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Jensen, Michael Krogh. (Editor), Keasling, Jay D. (Editor)
Format: eBook
Language:English
Published: New York, NY : Springer New York : Imprint: Humana, 2018.
Edition:1st ed. 2018.
Series:Methods in Molecular Biology, 1671
Subjects:
Table of Contents:
  • Parts characterization for tunable protein expression
  • Enzyme nicotinamide cofactor specificity reversal guided by automated structural analysis and library design
  • Bacterial genome editing strategy for control of transcription and protein stability
  • An Automated Pipeline for Engineering Many-Enzyme Pathways: Computational Sequence Design, Pathway Expression-Flux Mapping, and Scalable Pathway Optimization
  • Computational Approaches on Stoichiometric and Kinetic Modeling for Efficient Strain Design
  • Extended Metabolic Space Modeling
  • Computational Methods to Assess the Production
  • Designing and Implementing Algorithmic DNA Assembly Pipelines for Multi-gene Systems
  • An adaptive Laboratory Evolution Method to Accelerate Autotrophic Metabolism
  • CRISPR-Cas9 toolkit for Actinomycete Genome Editing
  • Assembly and Multiplex Genome Integration of Metabolic Pathways in Yeast using CasEMBLR
  • A modified Gibson assembly method for cloning large DNA fragments with high GC contents
  • Coupling Yeast Golden Gate and VEGAS for efficient assembly of the violacein pathway in Saccharomyces cerevisiae
  • Multi-capillary Column Ion Mobility Spectrometry of Volatile Metabolites For Phenotyping Of Microorganisms
  • Selection of Highly Expressed Gene Variants in Escherichia coli using Translationally-coupled Antibiotic Selection Markers
  • Design, Engineering, and Characterization Of Prokaryotic Ligand-binding Transcriptional Activators as Biosensors in Yeast
  • A Capture-SELEX Strategy for Multiplexed Selection of RNA Aptamers against Small Molecules
  • High-throughput Microfluidics for the Screening Of Yeast Libraries
  • Growth-Coupled Carotenoids Production Using Adaptive Laboratory Evolution
  • Two scale 13C Metabolic Flux Analysis for Metabolic Engineering.