Vaccine Adjuvants : Methods and Protocols - Fox, Christopher B.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
Overview of Vaccine Adjuvants: Introduction, History, and Current Status -- Development of the CpG Adjuvant 1018: A Case Study -- Syntheses of Human TLR8-Specific Small-Molecule Agonists -- Semisynthesis of Analogues of the Saponin Immunoadjuvant QS-21 -- QS-21 Adjuvant: Laboratory-Scale Purification Method and Formulation into Liposomes -- Purification of an Immunoadjuvant Saponin Fraction from Quillaja brasiliensis Leaves by Reverse-Phase Silica Gel Chromatography -- Biosynthetic Approaches to Squalene Production: The Case of Yeast -- In Silico Adjuvant Design and Validation -- Manufacturing Methods for Liposome Adjuvants -- Synthesis of Lymph Node-Targeting Adjuvants -- Preparing an Adjuvanted Thermoresponsive Gel Formulation for Sublingual Vaccination -- Manufacture of Oil-in-Water Emulsion Adjuvants -- Methods to Prepare Aluminum Salt-Adjuvanted Vaccines -- Production of Adjuvant-Loaded Biodegradable Particles for Use in Cancer Vaccines -- Lyophilization of Adjuvanted Vaccines: Methods for Formulation of a Thermostable Freeze Dried Product -- Stressed Stability Techniques for Adjuvant Formulations -- Particle Sizing of Nanoparticle Adjuvant Formulations by Dynamic Light Scattering (DLS) and Nanoparticle Tracking Analysis (NTA) -- Quantification of Multiple Components of Complex Aluminum-Based Adjuvant Mixtures by Using Fourier Transform Infrared Spectroscopy and Partial Least Squares Modeling -- Determination of Protein Content in Alhydrogelâ-Based Vaccines by O-Phthalaldehyde Assay -- Staining and Transfer Techniques for SDS-PAGE Gels to Minimize Oil-in-Water Emulsion Adjuvant Interference -- Interactions Between Antigens and Nanoemulsion Adjuvants: Separation and Characterization Techniques -- Screening Vaccine Formulations in Fresh Human Whole Blood -- Analysis of the Innate Response to Adjuvants: Characterization of the Draining Lymph Node by Fluorescence-Activated Cell Sorting -- Assessment of Antigen-Specific Cellular Immunogenicity Using Intracellular Cytokine Staining, ELISpot, and Culture Supernatants -- Eliciting Epitope-Specific CD8 T Cell Response by Immunization with Microbial Protein Antigens Formulated with α-Galactosylceramide: Theory, Practice, and Protocols -- Molecular Methods and Bioinformatic Tools for Adjuvant Characterization by High-Throughput Sequencing.
Keywords: Immunology; Immunology