The Genetic Manipulation of Staphylococci : Methods and Protocols - Bose, Jeffrey L.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2016)
Understanding Staphylococcal Nomenclature -- Restriction-Modification Systems as a Barrier for Genetic Manipulation of Staphylococcus aureus -- Complementation Plasmids, Inducible Gene-Expression Systems, and Reporters for Staphylococci -- De Novo Assembly of Plasmids Using Yeast Recombinational Cloning -- Splicing by Overlap Extension PCR to Obtain Hybrid DNA Products -- Method for Preparation and Electroporation of S. aureus and S. epidermidis -- Rapid Isolation of DNA from Staphylococcus -- Bacteriophage Transduction in Staphylococcus aureus: Broth-Based Method -- Bacteriophage Transduction in Staphylococcus aureus -- Batch Transduction of Transposon Mutant Libraries for Rapid Phenotype Screening in Staphylococcus -- Conjugative Transfer in Staphylococcus aureus -- Allelic Exchange -- Creation of Staphylococcal Mutant Libraries Using Transposon Tn917 -- Generation of a Transposon Mutant Library in Staphylococcus aureus and Staphylococcus epidermidis Using Bursa aurealis -- Chemical and UV Mutagenesis -- Pulsed Field Gel Electrophoresis -- RNA-Sequencing of Staphylococcus aureus Messenger RNA -- Quantitative Real-Time PCR (qPCR) Workflow for Analyzing Staphylococcus aureus Gene Expression -- Electrophoretic Mobility Shift Assays -- Rapid Amplification of cDNA Ends for RNA transcript Sequencing in Staphylococcus.
Keywords: Microbiology; Gene expression; Microbiology; Gene Expression