Techniques to Investigate Mitochondrial Function in Neurons - Strack, Stefan; Usachev, Yuriy M.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
Three-Dimensional Reconstruction of Neuronal Mitochondria by Electron Tomography -- Measuring Mitochondrial Shape with ImageJ -- Live Imaging Mitochondrial Transport in Neurons -- Techniques to Investigate Bioenergetics of Mitochondria -- Respirometry in Neurons -- Measuring ATP in Axons with FRET -- Characterizing Metabolic States using Fluorescence Lifetime Imaging Microscopy (FLIM) of NAD(P)H -- Techniques for Simultaneous Mitochondrial and Cytosolic Ca2+ Imaging in Neurons -- Live Imaging of Mitochondrial ROS Production and Dynamic Redox Balance in Neurons -- Monitoring of Permeability Transition Pore Openings in Isolated Individual Brain Mitochondria -- Examination of Mitochondrial Ion Conductance by Patch Clamp in Intact Neurons and Mitochondrial Membrane Preparations -- Monitoring of Permeability Transition Pore Openings in Mitochondria of Cultured Neurons -- Protocols for Assessing Mitophagy in Neuronal Cell Lines and Primary Neurons -- Examining Mitochondrial Function at Synapses In Situ -- Proteomic Analysis of Neuronal Mitochondria -- Measuring Mitochondrial Pyruvate Oxidation.
Keywords: Neurosciences; Neurosciences