The Golgi Complex : Methods and Protocols - Brown, William J.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2016)
4D Confocal Imaging of Yeast Organelles -- Imaging the Polarized Sorting of Proteins from the Golgi Complex in Live Neurons -- Imaging Golgi Outposts in Fixed and Living Neurons -- Analysis of Arf1 GTPase-Dependent Membrane Binding and Remodeling Using the Exomer Secretory Vesicle Cargo Adaptor -- STEM Tomography Imaging of Hypertrophied Golgi Stacks in Mucilage-Secreting Cells -- Reconstitution of COPI Vesicle and Tubule Formation -- Reconstitution of Phospholipase A2-Dependent Golgi Membrane Tubules -- Proteomic Characterization of Golgi Membranes Enriched from Arabidopsis Suspension Cell Cultures -- High Content Analysis of the Golgi Complex by Correlative Screening Microscopy -- Activity Detection of GalNAc Transferases by Protein-Based Fluorescence Sensors In Vivo -- In Situ Proximity Ligation Assay (PLA) Analysis of Protein Complexes Formed Between Golgi-Resident, Glycosylation-Related Transporters and Transferases in Adherent Mammalian Cell Cultures -- Creating Knock-Outs of Conserved Oligomeric Golgi Complex Subunits Using CRISPR-Mediated Gene Editing Paired with a Selection Strategy Based on Glycosylation Defects Associated with Impaired COG Complex Function -- Reversible Controlled Aggregation of Golgi Resident Enzymes to Assess Their Transport/Dynamics Along the Secretory Pathway -- Assays to Study the Fragmentation of the Golgi Complex During the G2/M Transition of the Cell Cycle -- The Role of Lysophospholipid Acyltransferases in the Golgi Complex -- Methods to Purify and Assay Secretory Pathway Kinases.
Keywords: Cell biology; Cell Biology