Strain Engineering : Methods and Protocols /
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2011.
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| Edition: | 1st ed. 2011. |
| Series: | Methods in Molecular Biology,
765 |
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Table of Contents:
- Bacterial Genome Reengineering
- Targeted Chromosomal Gene Knockout using PCR Fragments
- Scarless Chromosomal Gene Knockout Methods
- Random Chromosomal Gene Disruption In vivo using Transposomes
- Genome Engineering using Targeted Oligonucleotide Libraries and Functional Selection
- Microarray-based Genetic Footprinting Strategy to Identify Strain Improvement Genes after Competitive Selection of Transposon Libraries
- Optimization of Synthetic Operons using Libraries of Post-Transciptional Regulatory Elements
- Marker Free Chromosomal Expression of Foreign and Native Genes in E. coli
- Array-based Synthetic Genetic Screens to Map Bacterial Pathways and Functional Networks in Escherichia coli
- Assembling New E. coli Strains by Transduction using Phage P1
- Yeast Bioinformatics and Strain Engineering Resources
- Delete and Repeat: A Comprehensive Toolkit for Sequential Gene Knock-out in the Budding Yeast Saccharomyces cerevisiae
- Genome-wide Transposon Mutagenesis in Saccharomyces cerevisiae and Candida albicans
- Signature-tagged Mutagenesis to Characterize Genes Through Competitive Selection of Barcoded Genome Libraries
- Global Strain Engineering by Mutant Transcription Factors
- Genomic Promoter Replacement Cassettes to Alter Gene Expression in the Yeast Saccharomyces cerevisiae
- Microbial Genome Analysis and Comparisons: Web-based Protocols and Resources
- Plasmid Artificial Modification: A Novel Method for Efficient DNA Transfer into Bacteria
- Broad-host-range Plasmid Vectors for Gene Expression in Bacteria
- Simple Method for Introducing Marker-free Deletions in the Bacillus subtilis Genome
- Transposon-mediated Random Mutagenesis of Bacillus subtilis
- Integrative Food Grade Expression System for Lactic Acid Bacteria
- ClosTron-mediated Engineering of Clostridium
- High Throughput Transposon Mutagenesis of Corynebacterium glutamicum
- Mini-Mu Transposon Mutagenesis of Ethanologenic Zymomonas mobilis
- Engineering Thermoacidophilic Archaea using Linear DNA Recombination
- Targeted Gene Disruption in Koji Mold Aspergillus oryzae
- Selectable and Inheritable Gene Silencing through RNA Interference in the Unicellular Alga Chlamydomonas reinhardtii.