Therapeutic Proteins : Methods and Protocols - Voynov, Vladimir; Caravella, Justin A.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2012)
Therapeutic Proteins -- Synthetic Antibody Libraries -- The Construction of 'Phylomer' Peptide Libraries as a Rich Source of Potent Inhibitors of Protein/Protein Interactions -- Ribosome Display and Screening for Protein Therapeutics -- Yeast Display of Engineered Antibody Domains -- Design, Expression, Purification, and Characterization of Engineered Antibody CH2 and VH Domains -- Engineering of Affibody Molecules for Therapy and Diagnostics -- Protein Design for Diversity of Sequences and Conformations Using Dead-End Elimination -- Design and Generation of DVD-IgTM Molecules for Dual-Specific Targeting -- Engineering and Expression of Bibody and Tribody Constructs in Mammalian Cells and in the Yeast Pichia pastoris -- Use of E. coli for the Production of a Single Protein -- Folding Engineering Strategies for Efficient Membrane Protein Production in E. coli -- Transient Expression Technologies: Past, Present, and Future -- Stable Transfection Pools for Large Quantity of Protein Production -- Mammalian Stable Expression of Biotherapeutics -- Transgenic Expression of Therapeutic Proteins in Arabidopsis thaliana Seed -- Methods for Chromatographic Removal of Endotoxin -- Effectiveness of Various Processing Steps for Viral Clearance of Therapeutic Proteins: Database Analyses of Commonly-Used Steps -- High-Throughput Quantitative N-Glycan Analysis of Glycoproteins -- High-Throughput Multimodal Strong Anion Exchange Purification and N-Glycan Characterization of Endogenous Glycoprotein Expressed in Glycoengineered Pichia pastoris -- Databases and Tools in Glycobiology -- Characterization of PEGylated Biopharmaceutical Products by LC/MS and LC/MS/MS -- Identification of Asp Isomerization in Proteins by 18O Labeling and Tandem Mass Spectrometry -- Monitoring of Subvisible Particles in Therapeutic Proteins -- Size-Exclusion Chromatography with Multi-Angle Light Scattering (SEC-MALS) for Elucidating Protein Aggregation Mechanisms -- Computational Methods to Predict Therapeutic Protein Aggregation -- Coarse-Grained Simulations of Protein Aggregation -- Chitosan-Based Nanoparticles as Delivery Systems of Therapeutic Proteins -- Challenges in the Development and Manufacturing of Antibody-Drug Conjugates.
Keywords: Pharmacology; Proteomics; Pharmacology/Toxicology.; Proteomics