Two-Hybrid Systems : Methods and Protocols - Oñate-Sánchez, Luis.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2018)
Protein Interactomics by Two-Hybrid Methods -- Making the Right Choice: Critical Parameters of the Y2H Systems -- Identification of Protein-Protein Interactions Using Pool-Array-Based Yeast Two-Hybrid Screening -- Detecting Interactions of Membrane Proteins: The Split-Ubiquitin System -- The Ras Recruitment System (RRS) for the Identification and Characterization of Protein-Protein Interactions -- A Bacterial Adenylate Cyclase-Based Two-Hybrid System Compatible with Gateway® Cloning -- Analyses of Protein Interaction Networks Using Computational Tools -- Options and Considerations When Using a Yeast One-Hybrid System -- Screening Arrayed Libraries with DNA and Protein Baits to Identify Interacting Proteins -- Construction of Arabidopsis Transcription Factor ORFeome Collections and Identification of Protein-DNA Interactions by High-Throughput Yeast One-Hybrid Screens -- Transcription Factor-Centered Yeast One-Hybrid Assay -- The Yeast Three-Hybrid System for Protein Interactions -- The Yeast Three-Hybrid System for Screening RNA-Binding Proteins in Plants -- Using Yeast Hybrid System to Identify Proteins Binding to Small Molecules -- Shuttling of Entire Libraries from an Entry Vector to a Destination Vector of the Gateway System -- Bimolecular Fluorescence Complementation with Improved Gateway-Compatible Vectors to Visualize Protein-Protein Interactions in Plant Cells -- Bimolecular Fluorescence Complementation to Visualize Protein-Protein Interactions in Human Cells Based on Gateway Cloning Technology -- KISS: A Mammalian Two-Hybrid Method for In Situ Analysis of Protein-Protein Interactions -- Coimmunoprecipitation of Interacting Proteins in Plants -- Analysis of Protein-Protein Interaction by Co-IP in Human Cells -- Tandem Affinity Purification of Protein Complexes from Arabidopsis Cell Cultures -- Transient Transactivation Studies in Nicotiana benthamiana Leaves -- The Use of the Chromatin Immunoprecipitation Technique for In Vivo Identification of Plant Protein-DNA Interactions -- Chromatin Immunoprecipitation for Identification of Protein-DNA Interactions in Human Cells.
Keywords: Proteins .; Protein Science