T Cell Protocols : Development and Activation - Kearse, Kelly P.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2000)
Overview: For Chapters 2 - 15 -- Insights into T-Cell Development Using Transgenic and Knockout Mice -- T Cell Development -- Isolation and Characterization of Murine Early Intrathymic Precursor Populations -- Differentiation of Mouse Thymocytes in Fetal Thymus Organ Culture -- Purification of Immature CD4+CD8+ Thymocytes by Panning with Anti-CD8 Antibody -- Transfection and Transcription of Genes in Developing Thymocytes -- Analysis of CD4/CD8 Lineage Commitment by Pronase Treatment and Reexpression Assay -- Intrathymic Delivery of MHC Genes Using Recombinant Adenoviruses -- Quantitative Analysis of the Usage of Human T-Cell Receptor ? and ? Chain Variable Regions by Reverse Dot Blot Hybridization -- Flow Cytometric Detection of Cytoplasmic and Surface CD3-? Expression in Developing T Cells -- Assessing Apoptosis of Developing T Cells by Flow Cytometry -- Isolation of T-Cell Subsets by Magnetic Cell Sorting (MACS) -- Isolation of T-Cell Antigens by Retrovirus-Mediated Expression Cloning -- Flow Cytometric Analysis of Murine T Lymphocytes -- Production and Characterization of Immature Murine T-Lymphoma Cell Lines -- Production and Characterization of T-Cell Hybridomas -- Overview for Chapters 17-29 -- Something Happens; A Brief History of TCR Signal Transduction -- T-Cell Activation -- Measurement of Phosphatidylinositol (PI) Hydrolysis in Activated T Lymphocytes -- Fluorescence Polarization as an Early Measure of T-Lymphocyte Stimulation -- Measurement of Lymphoproliferation at the Single-Cell Level by Flow Cytometry -- Digital Image Analysis of Lymphocyte Activation -- Induction of T-Cell Signaling by Immobilized Integrin Ligands -- Cytotoxic T-Cell Adherence Assay (CAA) -- Detecting Ubiquitinated T-Cell Antigen Receptor Subunits by Immunoblotting -- Determination of CD45 Tyrosine Phosphatase Activity in T Lymphocytes -- Measurement of Protein Tyrosine Phosphorylation in T-Cell Subsets by Flow Cytometry -- Biochemical Analysis of Activated T Lymphocytes -- Activation of Heterotrimeric GTP-Binding Proteins Upon TCR/CD3 Engagement -- Generation of Human T Helper 1 and T Helper 2 Subsets from Peripheral Blood-Derived Naive CD4+ T Cells -- Transcriptional Expression Analysis of T-Cell Activation by Multiplex Messenger Assay (MMA).
Keywords: Immunology; Immunology