The Wilms' Tumor (WT1) Gene : Methods and Protocols - Hastie, Nicholas; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2016)
WT1 Mutation in Childhood Cancer -- WT1 and Renal Disease -- The Role of WT1 in Embryonic Development and Normal Organ Homeostasis -- Tools and Techniques for WT1-Based Lineage Tracing -- Biological Systems and Methods for Studying WT1 in the Epicardium -- Isolation and Colony Formation of Murine Bone and Bone Marrow Cells -- Isolation and Fluorescent Activated Cell Sorting of Murine WT1-Expressing Adipocyte Precursor Cells -- In Vivo Assays for Assessing the Role of the Wilms' Tumor Suppressor 1 (WT1) in Angiogenesis -- Multiphoton Microscopy for Visualizing Lipids in Tissue -- Function and Regulation of the Wilm's Tumor Suppressor 1 (WT1) Gene in Fish -- Immunofluorescence Staining of WT1 on Sections of Zebrafish Embryos and Larvae -- Fluorescence Activated Cell Sorting (FACS) Protocol for Podocyte Isolation in Adult Zebrafish -- In Vitro Transcription to Study WT1 Function -- Measuring Equilibrium Binding Constants for the WT1-DNA Interaction Using a Filter Binding Assay -- Identifying Direct Downstream Targets: WT1 ChIP-Seq Analysis -- WT1 Associated Protein-Protein Interaction Networks -- Methods to Identify and Validate WT1-RNA Interaction -- Bioinformatic Analysis of Next Generation Sequencing Data to Identify WT1 Associated Differential Gene and Isoform Expression -- Immunotherapy Targeting WT1: Designing a Protocol for WT1 Peptide-Based Cancer Vaccine.
Keywords: Cancer research; Human genetics; Cancer Research; Human Genetics