Rho GTPases : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Rivero, Francisco. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2012.
Edición:1st ed. 2012.
Colección:Methods in Molecular Biology, 827
Materias:
Tabla de Contenidos:
  • Historical Overview of Rho GTPases
  • Rho GTPases: Deciphering the Evolutionary History of a Complex Protein Family
  • Biochemical Assays to Characterize Rho GTPases
  • How to Analyze Bacterial Toxins Targeting Rho GTPases
  • Assessing Ubiquitylation of Rho GTPases in Mammalian Cells
  • Posttranslational Lipid Modification of Rho Family Small GTPases
  • Analysis of the Role of RhoGDI1 and Isoprenylation in the Degradation of Rho GTPases
  • A Quantitative Fluorometric Approach for Measuring the Interaction of RhoGDI with Membranes and Rho GTPases
  • Rho GTPases and Cancer Cell Transendothelial Migration
  • Quantitative and Robust Assay to Measure Cell-cell Contact Assembly and Maintenance
  • Rho GTPase Knockout Induction in Primary Keratinocytes from Adult Mice
  • Rho GTPase Techniques in Osteoclastogenesis
  • Assessment of Rho GTPase Signaling During Neurite Outgrowth
  • Assesment of the Role for Rho Family GTPases in NADPH Oxidase Activation
  • Multiplex Imaging of Rho GTPase Activities in Living Cells
  • FRET-Based Imaging of Rac and Cdc42 Activation During Fc-Receptor Mediated Phagocytosis in Macrophages
  • High Throughput Flow Cytometry Bead-Based Multiplex Assay for Identification of Rho GTPase Inhibitors
  • Functional Analysis of RhoGTPase Activation and Inhibition in a Bead-Based Miniaturized Format
  • Use of Phage Display For the Identification of Molecular Sensors Specific for Activated Rho
  • Identification of New Interacting Partners for Atypical Rho-GTPases: a SILAC-Based Approach
  • Using Zebrafish for Studying Rho GTPases Signaling in Vivo
  • Analysis of Rho GTPase Function in Axon Pathfinding Using Caenorhabditis elegans
  • Protocol for ex vivo Incubation of Drosophila Primary Post-Embryonic Haemocytes for Real-Time Analyses
  • Analysis of Rho GTPase Activation in Saccharomyces cerevisiae
  • Assaying Rho GTPase-Dependent Processes in Dictyostelium discoideum
  • FRAP-Based Analysis of Rho GTPase-dependent Polar Exocytosis in Pollen Tubes
  • Assaying Rho GTPase-Dependent Processes in Dictyostelium discoideum
  • FRAP-Based Analysis of Rho GTPase-dependent Polar Exocytosis in Pollen Tubes.