Transposons and Retrotransposons : Methods and Protocols - Garcia Perez, Jose Luis.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2016)
Study of Transposable Elements and their Genomic Impact -- Bacterial Group II Introns: Identification and Mobility Assay -- In silico Methods to Identify Exapted Transposable Element Families -- Retrotransposon Capture Sequencing (RC-seq): A Targeted, High-Throughput Approach to Resolve Somatic L1 Retrotransposition in Humans -- Long Interspersed Element Sequencing (L1-Seq): A Method to Identify Somatic LINE-1 Insertions in the Human Genome -- Combining Amplification Typing of L1 Active Subfamilies (ATLAS) with High Throughput Sequencing -- RNA-seq Analysis to Measure the Expression of SINE Retroelements -- Qualitative and Quantitative Assays of Transposition and Homologous Recombination of the Retrotransposon Tf1 in Schizosaccharomyces pombe -- LINE Retrotransposition Assays in Saccharomyces cerevisiae -- LINE-1 Cultured Cell Retrotransposition Assay -- L1 Retrotransposition in Neural Progenitor Cells -- Characterization of Engineered L1 Retrotransposition Events: The Recovery Method -- SINE Retrotransposition: Evaluation of Alu Activity and Recovery of de novo Inserts -- The Engineered SVA Trans-Mobilization Assay -- Detection of LINE-1 RNAs by Northern Blot -- Monitoring Long Interspersed Nuclear Element 1 Expression during Mouse Embryonic Stem Cells Differentiation -- Immuno-Detection of Human LINE-1 Expression in Cultured Cells and Human Tissues -- Cellular Localization of Engineered Human LINE-1 RNA and Proteins -- Purification of L1-ribonucleoprotein Particles (L1-RNPs) from Cultured Human Cells -- Characterization of L1-RiboNucleoprotein Particles -- LEAP: L1 Element Amplification Protocol -- Biochemical Approaches to Study LINE-1 Reverse Transcriptase Activity In Vitro -- Methylated DNA Immunoprecipitation Analysis of Mammalian Endogenous Retroviruses -- Profiling DNA Methylation and Hydroxymethylation at Retrotransposable Elements -- A Large-Scale Functional Screen to Identify Epigenetic Repressors of Retrotransposon Expression -- Reprogramming of Human Fibroblasts to Induced Pluripotent Stem Cells with Sleeping Beauty Transposon-based Stable Gene Delivery. .
Keywords: Human genetics; Human Genetics