Subtilisin Enzymes : Practical Protein Engineering - Bott, Richard; Betzel, Christian; SpringerLink (Online service) (New York, NY : Springer US : Imprint: Springer, 1996)
I. Structural Studies of Subtilisin Enzymes Alone and in Complex with Inhibitors -- 1. The Complex between Mesentericopeptidase and Eglin-C -- 2. Crystallographic Study of Eglin-C Binding to Thermitase -- 3. Design of Specific Peptide Structures and Subtilisin Enzyme Inhibitors Using ?, ß-Dehydro-Residues -- 4. Structure and Function of Subtilisin BPN' as Studied through Crystallographic Studies on a Series of Its Complexes with Genetically Engineered Proteinaceous Inhibitor SSI -- 5. Three Dimensional Structure of the Antibiotic Bacitracin A Complexed to Two Different Subtilisin Proteases: Novel Mode of Enzyme Inhibition -- 6. Active Site Binding Loop Stabilization in the Subtilisin Inhibitor Eglin C: Structural and Functional Studies on Specifically Designed Mutants in Complex with Subtilisin and the Uncomplexed Inhibitor -- 7. Crystal Strucutures of the Alkaline Proteases Savinase and Esperase from Bacillus lentus -- II. New Enzymes -- 8. Modelling and Engineering of Enzyme/Substrate Interactions in Subtilisin-like Enzymes of Unknown 3-Dimensional Structure -- 9. Substilases: Subtilisin-like Serine Proteases -- 10. Cloning and Expression of the Gene Encoding a Novel Proteinase from Tritirachium album Limber -- III. Structure-Function Studies -- 11. Studies of Binding Sites in the Subtilisin from Bacillus lentus by Means of Site Directed Mutagenesis and Kinetic Investigations -- 12. Laundry Performance of Subtilisin Proteases -- 13. Free Energy Perturbation Techniques Applied to Subtilisin BPN' Stability -- IV. Biophysical Probes and Mutagenesis -- 14. Thermitase-Kinetic Differentiation to the Subtilisins -- 15. Fluorescence Decay of Tryptophans in Serine Proteinases from Microorganisms: Relation to X-Ray Models -- 16. Propeptide-Mediated Folding in Subtilisin: The Intramolecular Chaperone Concept -- 17. Roles of the N- and C-Terminal Pro-Sequences of Aqualysin I Precursor in the Processing and Extracellular Secretion of the Enzyme -- 18. Crystal Structure Analysis of Subtilisin BPN' Mutants Engineered for Studying Thermal Stability -- 19. Random Mutagenesis of the Weak Calcium Binding Site in Subtilisin Carlsberg and Screening for Thermostability by Temperature-Gradient Gel Electrophoresis -- 20. Inhibition of Proteinase K by Mercury -- V. Protein Engineering of Subtilisin Enzymes -- 21. Crystallographic Investigations of Subtilisin BPN' Mutants Engineered for Studying Thermal Stability -- 22. Structural and Functional Consequences of Engineering the High Alkaline Serine Protease PB92 -- 23. Engineering Surface Charges in a Subtilisin -- 24. pH Dependence of the Catalytic Activity of a Subtilisin-like Proteinase -- 25. An Investigation of the Savinase Water Channel: Implications of Cavity Mutations -- 26. Substrate Specificity of Natural Variants and Genetically Engineered Intermediates of Bacillus lentus Alkaline Proteases -- 27. Unusual Ligand Binding at the Active Site Domain of an Engineered Mutant of Subtilisin BL -- 28. Studies on the Structure and Function of Subtilisin E by Protein Engineering -- 29. Structural Changes Leading to Increased Enzymatic Activity in an Engineered Variant of Bacillus lentus Subtilisin.
Keywords: Human genetics; Animal anatomy; Plant science; Botany; Biophysics; Biological physics; Human Genetics; Animal Anatomy / Morphology / Histology; Plant Sciences; Biological and Medical Physics, Biophysics