The Nuclear Envelope : Methods and Protocols - Shackleton, Sue.; Collas, Philippe; Schirmer, Eric C.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2016)
Isolation, Proteomic Analysis and Microscopy Confirmation of the Liver Nuclear Envelope Proteome -- Exploring the Protein Composition of the Plant Nuclear Envelope -- High Efficiency Isolation of Nuclear Envelope Protein Complexes from Trypanosomes -- Super Resolution Microscopy of the Nuclear Envelope and Associated Proteins -- Analyses of the Dynamic Properties of Nuclear Lamins by Fluorescence Recovery after Photobleaching (FRAP) and Fluorescence Correlation Spectroscopy (FCS) -- Probing Protein Distribution Along the Nuclear Envelope In Vivo by Using Single-Point FRAP -- The Use of 2-Photon FLIM/FRET to Study Protein Interactions during Nuclear Envelope Fusion In Vivo and In Vitro -- Identifying Protein-Protein Associations at the Nuclear Envelope with BioID -- In Situ Detection of Interactions between Nuclear Envelope Proteins and Partners -- Methods for Single-Cell Pulse-Chase Analysis of Nuclear Components -- Analysis of Nuclear Lamina Proteins in Myoblast Differentiation by Functional Complementation -- Analysis of Meiotic Telomere Behavior in the Mouse -- Identification and Validation of Putative Nesprin Variants -- Detection of Diverse and High Molecular Weight Nesprin-1 and Nesprin-2 Isoforms Using Western Blotting -- The Use of Polyacrylamide Hydrogels to Study the Effects of Matrix Stiffness on Nuclear Envelope Properties -- Cell Microharpooning to Study Nucleo-Cytoskeletal Coupling -- Wound Healing Assays to Study Mechanisms of Nuclear Movement in Fibroblasts and Myoblasts -- Methods for Assessing Nuclear Rotation and Nuclear Positioning in Developing Skeletal Muscle Cells -- Imaging Approaches to Investigate Myonuclear Positioning in Drosophila -- Mapping Nuclear Lamin-Genome Interactions by Chromatin Immunoprecipitation of Nuclear Lamins -- Lamin ChIP from Chromatin Prepared by Micrococcal Nuclease Digestion -- DamID Analysis of Nuclear Organization in Caenorhabditis elegans -- The Application of DamID to Identify Peripheral Gene Sequences in Differentiated and Primary Cells -- Visualizing the Spatial Relationship of the Genome with the Nuclear Envelope Using Fluorescence in situ Hybridization -- Visualization of Genomic Loci in Living Cells with a Fluorescent CRISPR/Cas9 System -- Methods to Monitor DNA Repair Defects and Genomic Instability in the Context of a Disrupted Nuclear Lamina -- High Resolution Scanning Electron Microscopy and Immuno-Gold Labeling of the Nuclear Lamina and Nuclear Pore Complex -- An In Vitro Assay to Study Targeting of Membrane Proteins to the Inner Nuclear Membrane -- Nuclear Protein Transport in Digitonin Permeabilized Cells -- Analysis of CRM1-Dependent Nuclear Export in Permeabilized Cells -- SPEED Microscopy and Its Application in Nucleocytoplasmic Transport.
Keywords: Cell biology; Cell Biology