Synapse Development : Methods and Protocols - Poulopoulos, Alexandros; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
A Cell Culture System to Investigate the Presynaptic Control of Subsynaptic Membrane Differentiation at the Neuromuscular Junction -- Co-Culture Synaptogenic Assay: A New Look at Fluorescence Reporters and Technological Devices -- Synaptogenic Assays Using Neurons Cultured on Micropatterned Substrates -- Monitoring Synapses Via Trans-Synaptic GFP Complementation -- Generation of Spinal Motor Neurons from Human Pluripotent Stem Cells -- Biochemical Purification of Binding Partners of Synaptic Scaffold Proteins -- In Situ Protein Binding Assay Using Fc-Fusion Proteins -- ation Microscopy for the Investigation of Synaptic Structure and Function -- 3D dSTORM Imaging of Fixed Brain Tissue -- Photomarking Relocalization Technique for Correlated Two-Photon and Electron Microcopy Imaging of Single Stimulated Synapses -- 3D Analysis of Synaptic Ultrastructure in Organotypic Hippocampal Slice Culture by High-Pressure Freezing and Electron Tomography -- Analyzing Endosomal Docking, Fusion, Sorting, and Budding Mechanisms in Isolated Organelles -- Concurrent Imaging of Receptor Trafficking and Calcium Dynamics by Spinning Disk Confocal Microscopy -- Imaging Activity-Dependent Signaling Dynamics at the Neuronal Synapse Using FRET-Based Biosensors -- Analyzing Structural Plasticity of Dendritic Spines in Organotypic Slice Culture -- Using Fluorescent Markers to Estimate Synaptic Connectivity In Situ -- Dual Anterograde and Retrograde Viral Tracing of Reciprocal Connectivity -- Mapping Synaptic Inputs of Developing Neurons Using Calcium Imaging -- Monosynaptic Tracing in Developing Circuits Using Modified Rabies Virus.
Keywords: Neurosciences; Neurosciences