Scanning Electron Microscopy of Cerebellar Cortex - Castejón, Orlando; SpringerLink (Online service) (New York, NY : Springer US : Imprint: Springer, 2003)
1. Sample Preparation Methods for Scanning Electron Microscopy -- Conventional SEM technique or slicing technique -- Fixation procedures -- The prefixation state of the nerve tissue -- Vascular perfusion fixation technique -- Criteria for good fixation and optimal preservation of nerve tissue -- Trimming procedure and obtaining nerve tissue slices -- Dehydration -- Critical point drying method -- Specimen mounting and orientation -- Metal deposition -- Nerve cell specimens coated with thick gold-palladium films -- Special SEM preparation techniques -- Concluding remarks -- 2. The Cerebellar White Matter -- Brief history -- The afferent and efferent fibers -- Concluding remarks -- 3. Granule Cells -- Short history -- The three-layered structure of cerebellar cortex -- Outer surface of intact granule cells -- Inner organization of fractured granule cells -- Granule cell processes -- Concluding remarks -- 4. The Mossy Fiber Glomerulus -- The long history -- The mossy fiber-granule cell synaptic relationship -- Concluding remarks -- 5. Golgi Cells -- Short history -- Scanning electron microscopy of unfractured Golgi cells -- Scanning electron microscopy of fractured Golgi cells -- Concluding remarks -- 6. Unipolar Brush Cells -- Recent history and three-dimensional morphology -- Future research -- 7. Lugaro Cells -- Short history -- Three-dimensional morphology -- Future research -- 8. Purkinje Cells -- The long history -- Three-dimensional morphology and outer surface -- Synaptic relationship with parallel fibers -- Concluding remarks -- 9. Climbing Fibers -- Brief history -- Intracortical course -- Concluding remarks -- 10. The Basket Cells -- Brief history -- Three-dimensional morphology -- Concluding remarks -- 11. Stellate Cells -- Brief history -- Three-dimensional morphology -- Concluding remarks -- 12. Cerebellar Glial Cells -- Brief history -- Oligodendrocytes -- The velate protoplasmic astrocytes -- Bergmann glial cells -- Concluding remarks -- 13. Cerebellar Capillaries -- Short history -- Three-dimensional morphology of cerebellar capillaries -- Contribution of SEM to the cerebellar blood-brain barrier structure and function -- Concluding remarks -- 14. Contribution of Scanning Electron Microscopy to Cerebellar Neurobiology -- The characterization of afferent and efferent fibers in the cerebellar white matter -- Three-dimensional visualization of unfractured and fractured neurons -- SEM as a high resolution tool for tracing cerebellar intracortical circuits -- The three-dimensional morphology of synaptic connections -- The three-dimensional morphology of glial cells -- Contribution to the information processing in the cerebellar cortex -- The three-dimensional design of cerebellar cortex -- References.
Keywords: Neurosciences; Microscopy; Neurosciences; Biological Microscopy