Signal Transduction Protocols - Kendall, David A.; Hill, Stephen J.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1995)
The Problems and Pitfalls of Radioligand Binding -- Solubilization and Purification of a Functional Ionotropic Excitatory Amino Acid Receptor -- Autoradiography of ?1-and ?2-Adrenoceptors -- In Situ Hybridization -- Measurement of the GTPase Activity of Signal-Transducing G-Proteins in Neuronal Membranes -- Measurement of Adenylyl Cyclase Activity in Cell Membranes -- The Measurement of Cyclic AMP Levels in Biological Preparations -- Mass Measurements of Cyclic AMP Formation by Radioimmunoassay, Enzyme Immunoassay, and Scintillation Proximity Assay -- Measurement of Cyclic GMP Formation -- Cyclic AMP-Dependent Protein Kinase Activity Ratio Assay -- Purification and Activation of Cyclic GMP-Dependent Protein Kinase -- The Analysis and Assay of Cyclic Nucleotide Phosphodiesterase Isoenzyme Activity -- Separation of Labeled Inositol Phosphate Isomers by High-Pressure Liquid Chromatography (HPLC) -- Mass Measurement of Key Phosphoinositide Cycle Intermediates -- Measurement of Phospholipase C Activity in Brain Membranes -- Measurement of Diacylglycerol -- Measurement of Intracellular Free Calcium Ion Concentration in Cell Populations Using Fura-2 -- Measurement of Ca2+ Fluxes in Permeabilized Cells Using 45Ca2+ and Fluo-3 -- Single-Cell Calcium Imaging -- Characterization of Calcium/Calmodulin-Stimulated Protein Kinase II -- Protein Kinase C -- The Measurement of Phospholipase D-Linked Signaling in Cells -- Phospholipase A2 Activity -- Techniques for the Measurement of Nitric Oxide.
Keywords: Cytology; Cell Biology