Rhodopsin : Methods and Protocols - Jastrzebska, Beata; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2015)
The G Protein-Coupled Receptor Rhodopsin: A Historical Perspective -- Rhodopsin Purification from Dark Adapted Bovine Retina -- Mammalian Expression, Purification, and Crystallization of Rhodopsin Variants -- Imaging of Rhodopsin Crystals with Two-Photon Microscopy -- Functional Stability of Rhodopsin in a Bicelle System: Evaluating G-Protein Activation by Rhodopsin in Bicelles -- The Rhodopsin-Arrestin-1 Interaction in Bicelles -- Detection of Structural Waters and Their Role in Structural Dynamics of Rhodopsin Activation -- Probing Conformational Changes in Rhodopsin Using Hydrogen-Deuterium Exchange Coupled to Mass Spectrometry -- Analysis of Conformational Changes in Rhodopsin by Histidine Hydrogen-Deuterium Exchange -- Investigation of Rhodopsin Dynamics in its Signaling State by Solid-State Deuterium NMR Spectroscopy -- Sequential Structural Changes in Rhodopsin Occurring upon Photoactivation -- Dynamic Single-Molecule Force Spectroscopy of Rhodopsin in Native Membranes -- High-Resolution Atomic Force Microscopy Imaging of Rhodopsin in Rod Outer Segment Disk Membranes -- Detection of Rhodopsin Dimerization in situ by PIE-FCCS, a Time-Resolved Fluorescence Spectroscopy -- Oligomeric State of Rhodopsin within Rhodopsin-Transducin Complex Probed with Succinylated Concanavalin A -- Quantification of Arrestin-Rhodopsin Binding Stoichiometry -- Rhodopsin Transient Complexes Investigated by Systems Biology Approaches -- Three Dimensional Architecture of Murine Rod Cilium Revealed by Cryo-EM -- Monitoring of Rhodopsin Trafficking and Mistrafficking in Live Photoreceptors -- Measurements of Rhodopsin Diffusion within Signaling Membrane Microcompartments in Live Photoreceptors -- Kinetics of Rhodopsin's Chromophore Monitored in a Single Photoreceptor -- Supplementation with Vitamin A Derivatives to Rescue Vision in Animal Models of Degenerative Retinal Diseases -- Sustained Delivery of Retinoids to Prevent Photoreceptor Death -- High-Throughput Screening Assays to Identify Small-Molecules Preventing Photoreceptor Degeneration Caused by the Rhodopsin P23H Mutation -- Gene Therapy to Rescue Retinal Degeneration Caused by Mutations in Rhodopsin.
Keywords: Cell biology; Proteomics; Cell membranes .; Cell Biology; Proteomics; Membrane Biology