Thermogenic Fat : Methods and Protocols - Wu, Jun.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
Isolation, Primary Culture, and Differentiation of Preadipocytes from Mouse Brown Adipose Tissue -- Isolation of Mouse Stromal Vascular Cells for Monolayer Culture -- Flow Cytometry Assisted Isolation of Adipose Tissue Derived Stem Cells -- Flow Cytometric Isolation and Differentiation of Adipogenic Progenitor Cells into Brown and Brite/Beige Adipocytes -- Immuno-Magnetic Isolation and Thermogenic Differentiation of White Adipose Tissue Progenitor Cells -- Isolation of Immune Cells from Adipose Tissue -- Differentiation and Metabolic Interrogation of Human Adipocytes -- Protocols for Generation of Immortalized Human Brown and White Preadipocyte Cell Lines -- Gene Expression and Histological Analysis of Activated Brown Adipocytes in Adipose Tissue -- Genetic Mouse Models: The Powerful Tools to Study Fat Tissues -- Genetic Manipulation with Viral Vectors to Assess Metabolism and Adipose Tissue Function -- Bioenergetic Analyses in Adipose Tissue -- Oxygen Consumption Rate and Energy Expenditure in Mice: Indirect Calorimetry -- Isolation and Patch-Clamp of Primary Adipocytes -- In Vitro Approaches to Model and Study Communications Between Adipose Tissue and the Liver -- Identification and Quantification of Human Brown Adipose Tissue -- Designing 3D Adipospheres for Quantitative Metabolic Study -- Culture and Sampling of Primary Adipose Tissue in Practical Microfluidic Systems -- Using Thermogenic Beige Cells to Identify Biologically Active Small Molecules and Peptides.
Keywords: Cell biology; Cell Biology