Sirtuins : Methods and Protocols - Hirschey, Matthew D.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2013)
Introduction: Sirtuins in Aging and Diseases -- Sirtuins in Yeast: Phenotypes and Tools -- Studying the C. elegans Sirtuins -- Genetic and Biochemical Tools for Investigating Sirtuin Function in Drosophila melanogaster -- Generating Mammalian Sirtuin Tools for Protein-Interaction Analysis -- Mass Spectrometry-Based Detection of Protein Acetylation -- SILAC-Based Quantification of Sirt1-Responsive Lysine Acetylome -- Targeted Quantitation of Acetylated Lysine Peptides by Selected Reaction Monitoring Mass Spectrometry -- Identification of Deacetylase Substrates with the Biotin Switch Approach -- Assaying Chromatin Sirtuins -- Measurement of Sirtuin Enzyme Activity Using a Substrate-Agnostic Fluorometric Nicotinamide Assay -- Detecting Sirtuin-Catalyzed Deacylation Reactions Using 32P-Labeled NAD and Thin-Layer Chromatography -- Chemical Acetylation And Deacetylation -- Accurate Measurement of Nicotinamide Adenine Dinucleotide (NAD+) with High-Performance Liquid Chromatography -- In Vivo Measurement of the Acetylation State of Sirtuin Substrates as a Proxy for Sirtuin Activity -- Oxygen Flux Analysis to Understand the Biological Function of Sirtuins -- The Emerging Links Between Sirtuins and Autophagy -- Methods to Study the Role of Sirtuins in Genome Stability -- Circadian Measurements of Sirtuin Biology -- Utilizing Calorie Restriction to Evaluate the Role of Sirtuins in Healthspan and Lifespan of Mice.
Keywords: Animal genetics; Proteomics; Animal Genetics and Genomics; Proteomics