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161111s2017 xxu| s |||| 0|eng d |
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|a 9781493966493
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| 024 |
7 |
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|a 10.1007/978-1-4939-6649-3
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Type 3 Secretion Systems :
|b Methods and Protocols /
|c edited by Matthew L. Nilles, Danielle L. Jessen Condry.
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| 250 |
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|a 1st ed. 2017.
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| 260 |
# |
# |
|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2017.
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| 300 |
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|a X, 224 p. 32 illus., 23 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1531
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| 505 |
0 |
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|a Introduction to Type III Secretion Systems -- Site-Directed Mutagenesis and its Application in Studying the Interactions of T3S Components -- Blue Native Protein Electrophoresis to Study the T3S System using Yersinia pestis as a Model -- In Vivo Photo-Cross-Linking to Study T3S Interactions Demonstrated using the Yersinia pestis T3S System -- Isolation of Type III Secretion System Needle Complexes by Shearing -- Use of Transcriptional Control to Increase Expression and Secretion of Heterologous Proteins in T3S Systems -- Characterization of Type Three Secretion System Translocator Interactions with Phospholipid Membranes -- Analysis of Type III Secretion System Secreted Proteins -- Fractionation Techniques to Examine Effector Translocation -- Measurement of Effector Protein Translocation using Phosphorylatable Epitope Tags and Phospho-Specific Antibodies -- A TAL-Based Reporter Assay for Monitoring Type III-Dependent Protein Translocation in Xanthomonas -- Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in Plants -- A Method for Characterizing the Type III Secretion System’s Contribution to Pathogenesis: Homologous Recombination to Generate Yersinia pestis Type III Secretion System Mutants -- Detecting Immune Responses to Type III Secretion Systems -- Recombinant Expression and Purification of the Shigella Translocator IpaB -- Expression and Purification of N-Terminally His-Tagged Recombinant Type III Secretion Proteins -- Mouse Immunization with Purified Needle Proteins from Type III Secretion Systems and the Characterization of the Immune Response to these Proteins -- Identification of the Targets of Type III Secretion System Inhibitors -- Detection of Protein Interactions in T3S Systems using Yeast 2-Hybrid Analysis.
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| 650 |
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0 |
|a Microbiology.
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| 650 |
1 |
4 |
|a Microbiology.
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| 700 |
1 |
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|a Nilles, Matthew L.
|e editor.
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| 700 |
1 |
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|a Condry, Danielle L. Jessen.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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