Type 3 Secretion Systems : Methods and Protocols - Nilles, Matthew L.; Condry, Danielle L. Jessen; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
Introduction to Type III Secretion Systems -- Site-Directed Mutagenesis and its Application in Studying the Interactions of T3S Components -- Blue Native Protein Electrophoresis to Study the T3S System using Yersinia pestis as a Model -- In Vivo Photo-Cross-Linking to Study T3S Interactions Demonstrated using the Yersinia pestis T3S System -- Isolation of Type III Secretion System Needle Complexes by Shearing -- Use of Transcriptional Control to Increase Expression and Secretion of Heterologous Proteins in T3S Systems -- Characterization of Type Three Secretion System Translocator Interactions with Phospholipid Membranes -- Analysis of Type III Secretion System Secreted Proteins -- Fractionation Techniques to Examine Effector Translocation -- Measurement of Effector Protein Translocation using Phosphorylatable Epitope Tags and Phospho-Specific Antibodies -- A TAL-Based Reporter Assay for Monitoring Type III-Dependent Protein Translocation in Xanthomonas -- Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in Plants -- A Method for Characterizing the Type III Secretion System's Contribution to Pathogenesis: Homologous Recombination to Generate Yersinia pestis Type III Secretion System Mutants -- Detecting Immune Responses to Type III Secretion Systems -- Recombinant Expression and Purification of the Shigella Translocator IpaB -- Expression and Purification of N-Terminally His-Tagged Recombinant Type III Secretion Proteins -- Mouse Immunization with Purified Needle Proteins from Type III Secretion Systems and the Characterization of the Immune Response to these Proteins -- Identification of the Targets of Type III Secretion System Inhibitors -- Detection of Protein Interactions in T3S Systems using Yeast 2-Hybrid Analysis.
Keywords: Microbiology; Microbiology