Telomeres and Telomerase : Methods and Protocols - Songyang, Zhou.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
Introduction to Telomeres and Telomerase -- Analysis of Average Telomere Length in Human Telomeric Protein Knockout Cells Generated by CRISPR/Cas9 -- Telomere Length Analysis by Quantitative Fluorescent In Situ Hybridization (Q-FISH) -- Telomere Strand-Specific Length Analysis by Fluorescent In Situ Hybridization (Q-CO-FISH) -- Telomere G-Rich Overhang Length Measurement: DSN Method -- Telomere G-Overhang Length Measurement Method 2: G-Tail Telomere HPA -- Telomere Terminal G/C Strand Synthesis: Measuring Telomerase Action and C-Rich Fill-In -- Analysis of Yeast Telomerase by Primer Extension Assays -- Assessing Telomerase Activities in Mammalian Cells Using the Quantitative PCR-Based Telomeric Repeat Amplification Protocol (qTRAP) -- Telomeres and NextGen CO-FISH: Directional Genomic Hybridization (Telo-dGH™) -- Visualization of Human Telomerase Localization by Fluorescence Microscopy Techniques -- Cytogenetic Analysis of Telomere Dysfunction -- Probing the Telomere Damage Response -- Induction of Site-Specific Oxidative Damage at Telomeres by Killerred-Fused Shelretin Proteins -- Using Protein-Fragment Complementation Assays (PCA) and Peptide Arrays to Study Telomeric Protein-Protein Interactions -- In Vitro Preparation and Crystallization of Vertebrate Telomerase Subunits -- Human Telomeric G-Quadruplex Structures and G-Quadruplex-Interactive Compounds -- Analysis of Telomere-Homologous DNA with Different Conformations Using 2D Agarose Electrophoresis and In-Gel Hybridization -- Analysis of Telomere Proteins by Chromatin Immunoprecipitation (ChIP). .
Keywords: Biochemistry; Biochemistry, general