Schwann Cells : Methods and Protocols - Monje, Paula V.; Kim, Haesun A.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2018)
Isolation of Schwann Cell Precursors from Rodents -- Preparation of Neonatal Rat Schwann cells and Embryonic Dorsal Root Ganglia Neurons for In Vitro Myelination Studies -- Isolation and Expansion of Schwann Cells from Transgenic Mouse Models -- Isolation, Culture, and Cryopreservation of Adult Rodent Schwann Cells Derived from Immediately Dissociated Teased Fibers -- Detailed Protocols for the Isolation, Culture, Enrichment, and Immunostaining of Primary Human Schwann cells -- Magnetic-activated Cell Sorting for the Fast and Efficient Separation of Human and Rodent Schwann Cells from Mixed Cell Populations -- Culture and Expansion of Rodent and Porcine Schwann Cell for Pre-clinical Animal Studies -- Chemical Conversion of Human Fibroblasts into functional Schwann Cells -- Derivation of Fate-committed Schwann Cells from Bone Marrow Stromal Cells of Adult Rats -- Human Induced Pluripotent Stem Cell (hiPS cell)-derived Sensory Neurons for Fate Commitment of Bone Marrow Stromal Cell-derived Schwann Cells -- Generation and Use of Merlin-Deficient Human Schwann Cells for a High-Throughput Chemical Genomics Screening Assay -- Lentiviral Transduction of Rat Schwann Cells and Dorsal Root Ganglia Neurons for In Vitro Myelination Studies -- Preservation, Sectioning and Staining of Schwann Cell Cultures for Transmission Electron Microscopy Analysis -- Scalable Differentiation and Dedifferentiation Assays using Neuron-free Schwann Cell Cultures -- The Pseudopod System for Axon-glia Interactions: Stimulation and Isolation of Schwann cell Protrusions that form in Response to Axonal Membranes -- In vitro Analysis of the Role of Schwann Cells on Axonal Degeneration and Regeneration using Sensory Neurons from Dorsal Root Ganglia -- A Culture Model To Study Neuron - Schwann cell - Astrocyte Interactions -- Preparation of Matrices of Variable Stiffness for the Study of Mechanotransduction in Schwann Cell Development -- Purification of Exosomes from Primary Schwann cells, RNA Extraction and Next Generation Sequencing of Exosomal RNAs -- 3D Cancer Migration Assay with Schwann Cells -- Teased Fiber Preparation of Myelinated Nerve Fibers from Peripheral Nerves for Vital Dye Staining and Immunofluorescence Analysis -- Whole Mount Immunostaining on Mouse Sciatic Nerves to Visualize Events of Peripheral Nerve Regeneration -- The use of Low Vacuum Scanning Electron Microscopy (LVSEM) to Analyze Peripheral Nerve Myelin -- Analysis of Myelinating Schwann Cells in Human Skin Biopsies -- Whole Mount In Situ Hybridization and Immunohistochemistry for Myelin in Zebra Fish Larvae -- Transmission Electron Microscopy for Zebrafish Larvae and Adult Lateral Line Nerve to Visualize Myelin -- Live Imaging of Schwann Cell Development in Zebra Fish -- Transplantation of Adult Rat Schwann Cells into the Injured Spinal Cord -- Schwann Cell Transplantation Methods Using Biomaterials -- Viral Transduction of Schwann Cells for Peripheral Nerve Repair -- Intraspinal Delivery of Schwann Cells for Spinal Cord Injury. .
Keywords: Cell biology; Cell Biology