Type 2 Immunity : Methods and Protocols - Reinhardt, R. Lee.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2018)
A Fungal Protease Model To Interrogate Allergic Lung Immunity -- Use of the Litomosoides sigmodontis Infection Model of Filariasis to study Type 2 Immunity -- Production of Hymenolepis diminuta in the Laboratory: An Old Research Tool With New Clinical Applications -- A Mouse Model of Peanut Allergy Induced By Sensitization through the Gastrointestinal Tract -- Induction and Characterization of the Allergic Eye Disease Mouse Model -- Isolation and Purification of Epithelial and Endothelial Cells from Mouse Lung -- In vitro and In vivo IgE/Antigen Mediated Mast Cell Activation -- The use of Human and Mouse Mast Cell and Basophil Cultures to Assess type-2 Inflammation -- Isolation and Identification of Group 2 Innate Lymphoid Cells in Settings of Type 2 Inflammation -- Determination of the Fate and Function of Innate Lymphoid Cells Following Adoptive Transfer of Innate Lymphoid Cell Precursors -- Characterization of Thymic Development of Natural Killer T Cell Subsets by Multiparameter Flow Cytometry -- Characterization of Mouse gd T Cell Subsets in the Setting of type-2 Immunity -- The Identification of Allergen-derived T cell Epitopes -- Generation of Allergen-specific Tetramers for a Murine Model of Airway Inflammation -- The Generation and Use of Allergen-specific TCR Transgenic Animals -- Using Cytokine Reporter Mice to Visualize Type-2 immunity in vivo -- Live Imaging of IL-4 expressing T Follicular Helper Cells in Explanted Lymph Nodes -- Imaging Precision Cut Lung Slices to Visualize Leukocyte Localization and Trafficking -- Study of IgE-producing B cells using the Verigem Fluorescent Reporter Mouse -- Chromatin Preparation from Murine Eosinophils for Genome-wide Analyses -- A Sensitive and Integrated Approach to Profile Messenger RNA from Samples with Low Cell Numbers -- An Integrated and Semi-automated Micro-scaled Approach to Profile Cis-Regulatory Elements by Histone Modification ChIP-Seq for Large-scale Epigenetic Studies -- Library preparation for ATAC-sequencing of Mouse CD4+ T Cells Isolated from the Lung and Lymph Nodes after Helminth Infection -- Identification of Functionally-relevant microRNAs in the Regulation of Allergic Inflammation -- The Use of Biodegradable Nanoparticles for Tolerogenic Therapy of Allergic Inflammation -- Assessing the Mouse Intestinal Microbiota in Settings of type-2 Immune Responses -- The use of CRISPR-Cas9 Technology to Reveal Important Aspects of Human Airway Biology -- A Consistent Method to Identify and Isolate Mononuclear Phagocytes from Human Lung and Lymph Nodes -- Organoid Cultures for Assessing Intestinal Epithelial Differentiation and Function in Response to Type-2 Inflammation -- Utilization of Air-liquid Interface Cultures as an in vitro Model to Assess Primary Airway Epithelial Cell Responses to the Type 2 Cytokine Interleukin 13.
Keywords: Immunology; Immunology