The Complement System : Methods and Protocols - Gadjeva, Mihaela; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2014)
Overview -- Evaluation of Complement Function by ELISA -- Classical Complement Pathway Component C1q - Purification of Human C1q, Isolation of C1q Collagen-like and Globular Head Fragments and Production of Recombinant C1q - Derivatives. Functional Characterization -- Classical Complement Pathway Components C1r and C1s - Purification from Human Serum and in Recombinant Form and Functional Characterization -- Expression and Purification Methods for the Production of Recombinant Human Complement Component C2 -- Purification and Characterisation of human and Mouse Complement C3 -- Purification of Human Complement Protein C5 -- Quantification of Complement C5b-9 Binding to Cells by Flow Cytometry -- Challenges and Opportunities in Fractionation of Recombinant Human Mannan-binding Lectin -- Genotyping of FCN and MBL2 Polymorphisms using Pyrosequencing -- Assay for Estimation of the Functional Activity of the Mannan Binding Lectin Pathway of the Complement System -- Purification, Measurement of Concentration, and Functional Complement Assay of Human Ficolins -- Purification and Functional Analysis of Human Properdin -- Purification and Functional Characterization of C4b-binding Protein (C4BP) -- Purification and Functional Characterization of Factor I -- C1 inhibitor - Quantification and Purification -- Purification, Quantification and Functional Analysis of Complement Factor H -- Factor H-related Proteins -- Functional Evaluation of Factor H genetic and Acquired Abnormalities; Application for Atypical Hemolytic Uremic Syndrome (aHUS) -- Anti-Factor H Auto Antibodies Assay -- Purification of Native Surfactant Protein SP-A from Pooled Amniotic Fluid and Bronchoalveolar Lavage -- Purification of Surfactant Protein D (SP- D) from Pooled Amniotic Fluid and Bronchoalveolar Lavage -- Functional Analysis of C5a Effector Responses In Vitro and In Vivo -- Detection of Complement Receptors 1 and 2 on Mouse Splenic B Cells using Flow Cytometry -- Quantification of Complement Receptor 2 Calcium Signaling Enhancement Using Flow Cytometry -- Purification of C1q Receptors and Functional Analysis -- Detection of Cell Membrane-bound CD46 using Flow Cytometry -- Detection of CD59 using Flow Cytometry -- Functional Analysis of CD59 using Complement Dependent Cytotoxicity Assay -- Detection of Soluble CR3 (CD11b/CD18) by Time-resolved Immunofluorometry -- Analysis of the Complement Activation in Mice -- Opsonophagocytic Assay.
Keywords: Immunology; Antibodies; Immunology; Antibodies