Sertoli Cells : Methods and Protocols - Alves, Marco G.; Oliveira, Pedro F.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2018)
Establishment of Primary Culture of Sertoli Cells -- Evaluation of the Purity of Sertoli Cell Primary Cultures -- Preparation of Testicular Samples for Histology and Immunohistochemistry -- Rabbit Sertoli Cells: Immunohistochemical Profile from Neonatal to Adult Age -- Identification of Proliferative and Apoptotic Sertoli Cells Using Fluorescence and Confocal Microscopy -- Sertoli Cells Preparation for Co-Immunoprecipitation -- Profiling Signaling Proteins in Sertoli Cells by Co-Immunoprecipitation -- Phagocytosis by Sertoli Cells: Analysis of Main Phagocytosis Steps by Confocal and Electron Microscopy -- A Method for In Vivo Induction and Ultrastructural Detection of Mitophagy in Sertoli Cells -- Assessing Autophagy in Sertoli Cells -- Molecular Mechanisms and Signaling Pathways Involved in the Nutritional Support of Spermatogenesis by Sertoli Cells -- Assessing Sertoli Cells Metabolic Activity -- Proteome Profiling of Sertoli Cells Using a GeLC-MS/MS Strategy -- Gene Silencing of Human Sertoli Cells Utilizing Small Interfering RNAs -- Testicular Cell Selective Ablation Using Diphtheria Toxin Receptor Transgenic Mice -- Regulation of Blood-Testis Barrier (BTB) Dynamics, Role of the Actin, and Microtubule-Based Cytoskeletons -- Monitoring the Integrity of the Blood-Testis Barrier (BTB): An In Vivo Assay -- Computational Methods Involved in Evaluating the Toxicity of the Reproductive Roxicants in Sertoli Cell -- A Stopped-Flow Light Scattering Methodology for Assessing the Osmotic Water Permeability of Whole Sertoli Cells -- Cryopreservation of Human Testicular Tissue by Isopropyl-Controlled Slow Freezing.
Keywords: Cell biology; Cell Biology