Sphingosine-1-Phosphate : Methods and Protocols - Pébay, Alice; Turksen, Kursad; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2012)
Biochemical Methods for Quantifying Sphingolipids: Ceramide, Sphingosine, Sphingosine Kinase-1 Activity, and Sphingosine-1-Phosphate -- Isoform-Selective Assays for Sphingosine Kinase Activity -- Quantification of Sphingosine-1-Phosphate and Related Sphingolipids by Liquid Chromatography Coupled to Tandem Mass Spectrometry -- A Clean-up Method for Mass Spectrometric Analysis of Sphingosine-1-Phosphate in Blood and Solid Tissues Using a Phosphate Capture Molecule -- Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Samples -- Assessment of Sphingosine-1-Phosphate Activity in Biological Samples by Receptor Internalization and Adherens Junction Formation -- High Throughput Assays to Measure Intracellular Ca2+ Mobilization in Cells that Express Recombinant S1P Receptor Subtypes -- Quantifying Sphingosine-1-Phosphate-Dependent Activation of the RhoGTPases -- Optimized Protocols to Analyze Sphingosine-1-Phosphate Signal Transduction Pathways During Acrosomal Exocytosis in Human Sperm -- Use of Intravital Microscopy and In Vitro Chemotaxis Assays to Study the Roles of Sphingosine-1-Phosphate in Bone Homeostasis -- Assessment of Sphingosine-1-Phosphate Receptor Expression and Associated Intracellular Signaling Cascades in Primary Cells of the Human Central Nervous System -- Sphingosine-1-Phosphate Signaling in Skeletal Muscle Cells -- Maintenance of Human Embryonic Stem Cells by Sphingosine-1-Phosphate and Platelet-Derived Growth Factor -- Ceramide and Sphingosine-1-Phosphate Signaling in Embryonic Stem Cell Differentiation -- Sphingosine-1-Phosphate: An Alternative to Serum for Culture and Multipotency Maintenance of Adult Human Mesenchymal Stem Cells -- Sphingosine-1-Phosphate Signaling in Neural Progenitors -- Utilizing Sphingosine-1-Phosphate to Stimulate Sprouting Angiogenesis.
Keywords: Cell biology; Cell culture; Cell Biology; Cell Culture