Structural Genomics and Drug Discovery : Methods and Protocols - Anderson, Wayne F.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2014)
Data Management in the Modern Structural Biology and Biomedical Research Environment -- Structural Genomics of Human Proteins -- Target Selection for Structural Genomics of Infectious Diseases -- Selecting Targets from Eukaryotic Parasites for Structural Genomics and Drug Discovery -- High Throughput Cloning for Biophysical Applications -- Expression and Solubility Testing in a High Throughput Environment -- Protein Production for Structural Genomics Using E. coli Expression -- Eukaryotic Expression Systems for Structural Studies -- Automated Cell-free Protein Production Methods for Structural Studies -- Parallel Protein Purification -- Oxidative Refolding from Inclusion Bodies -- High throughput Crystallization Screening -- Screening Proteins for NMR Suitability -- Salvage or Recovery of Failed Targets by in situ Proteolysis -- Salvage of Failed Protein Targets by Reductive Alkylation -- Salvage or Recovery of Failed Targets by Mutagenesis to Reduce Surface Entropy -- Data Collection for Crystallographic Structure Determination -- Structure, Determination, Refinement, and Validation -- Virtual High-Throughput Ligand Screening -- Ligand Screening using Fluorescence Thermal Shift Analysis (FTS) -- Ligand Screening using Enzymatic Assays -- Ligand Screening using NMR -- Screening Ligands by X-ray Crystallography -- Case Study - Structural Genomics and Human Protein Kinases.
Keywords: Pharmacology; Human genetics; Pharmacology/Toxicology.; Human Genetics