Signal Transduction Protocols - Luttrell, Louis M.; Ferguson, Stephen S. G.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2011)
Refining Efficacy: Allosterism and Bias in G Protein-Coupled Receptor Signaling -- Imaging-based Approaches to Understanding G Protein-Coupled Receptor Signaling Complexes -- Improving Drug Discovery with Contextual Assays and Cellular Systems Analysis -- RGS-insensitive Ga Subunits: Probes of Ga Subtype-selective Signaling and Physiological Functions of RGS Proteins -- Bioinformatic Approaches to Metabolic Pathways Analysis -- Studying Ligand Efficacy at G Protein-Coupled Receptors Using FRET -- Using BRET to Detect Ligand-Specific Conformational Changes in Preformed Signaling Complexes -- Reconstitution of G Protein-Coupled Receptors into a Model Bilayer System: Reconstituted High Density Lipoprotein Particles (rHDL) -- Using Quantitative BRET to Assess G Protein-Coupled Receptor Homo- and Hetero-Dimerization -- Cell-Surface Protein-Protein Interaction Analysis with Time-Resolved FRET and Snap-tag Technologies: Application to G Protein-Coupled Receptor Oligomerization -- Analysis of GPCR/Ion Channel Interactions -- Multicolor BiFC Analysis of G Protein bg Complex Formation and Localization -- Real-Time BRET Assays to Measure G Protein/Effector Interactions -- Luminescent Biosensors for Real-Time Monitoring of Intracellular cAMP -- Simultaneous Real Time Imaging of Signal Oscillations Using Multiple Fluorescence-Based Reporters -- Using FRET-based Reporters to Visualize Subcellular Dynamics of Protein Kinase A Activity -- Genetically Encoded Fluorescent Reporters to Visualize Protein Kinase C Activation in Live Cells -- Visualizing Receptor Endocytosis and Trafficking -- Investigating G Protein-Coupled Receptor Endocytosis and Trafficking by TIR-FM -- Visualizing G Protein-Coupled Receptor Signalsomes Using Confocal Immunofluorescence Microscopy -- Detection and Characterization of Receptor Interactions with PDZ Domains -- Tandem Affinity Purification and Identification of Heterotrimeric G-Protein Associated Proteins -- Study of G Protein-Coupled Receptor/ßarrestin Interactions within Endosomes Using FRAP -- Disrupting Protein Complexes Using Tat-tagged Peptide Mimics -- Protein-fragment Complementation Assays for Large-Scale Analysis, Functional Dissection and Dynamic Studies of Protein-Protein Interactions in Living Cells.
Keywords: Cytology; RNA-ligand interactions; Cell Biology; Protein-Ligand Interactions