T-Cell Trafficking : Methods and Protocols - Marelli-Berg, Federica M.; Nourshargh, Sussan; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2010)
Introductory Review -- How T Cells Find Their Way Around -- Migration of T Cells In Vitro -- Live Imaging of Leukocyte-Endothelium Interactions -- Leucocyte Adhesion Under Haemodynamic Flow Conditions -- Influence of Stromal Cells on Lymphocyte Adhesion and Migration on Endothelial Cells -- Discriminating Between the Paracellular and Transcellular Routes of Diapedesis -- Monitoring RhoGTPase Activity in Lymphocytes -- Visualisation of Signalling in Immune Cells -- Methods for Quantitation of Leukocyte Chemotaxis and Fugetaxis -- Analysis of CXCR3 and Atypical Variant Expression and Signalling in Human T Lymphocytes -- Transfection of Indoleamine 2,3 Dioxygenase in Primary Endothelial Cells -- Migration of T Cells In Vivo -- Visualisation of Lymphoid Organ Development -- Single-Cell Analysis of Cytotoxic T Cell Function by Intravital Multiphoton Microscopy -- Imaging Interactions Between the Immune and Cardiovascular Systems In Vivo by Multiphoton Microscopy -- Applying an Adaptive Watershed to the Tissue Cell Quantification During T-Cell Migration and Embryonic Development -- Monitoring T-Cell Migration in Human Diseases -- Identifying Homing Interactions in T-Cell Traffic in Human Disease -- Tracking Antigen-Experienced Effector T Cells In Vitro and In Vivo -- Preclinical Testing of Strategies for Therapeutic Targeting of Human T-Cell Trafficking In Vivo.
Keywords: Immunology; Laboratory medicine; Immunology; Laboratory Medicine