Spliceosomal Pre-mRNA Splicing : Methods and Protocols - Hertel, Klemens J.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2014)
The Pre-mRNA Splicing Reaction -- Diversity and Evolution of Spliceosomal Systems -- Mechanisms of Spliceosomal Assembly -- Alternative Pre-mRNA Splicing -- Regulation of Alternative Pre-mRNA Splicing -- Introduction to Co-Transcriptional RNA Splicing -- Chromatin and Splicing -- Preparation of Splicing Competent Nuclear Extracts -- Preparation of Yeast Whole Cell Splicing Extract -- Efficient Splinted Ligation of Synthetic RNA Using RNA Ligase -- In Vitro Assay of Pre-mRNA Splicing in Mammalian Nuclear Extract -- Kinetic Analysis of In Vitro Pre-mRNA Splicing in HeLa Nuclear Extract -- In Vitro Systems for Coupling RNAP II Transcription to Splicing and Polyadenylation -- Isolation and Accumulation of Spliceosomal Assembly Intermediates -- Complementation of U4 snRNA in S. cerevisiae Splicing Extracts for Biochemical Studies of snRNP Assembly and Function -- Expression and Purification of Splicing Proteins from Mammalian Cells -- Single Molecule Approaches for Studying Spliceosome Assembly and Catalysis -- Cell-Based Splicing of Minigenes -- Quantifying the Ratio of Spliceosome Components Assembled on Pre-mRNA -- Antisense Methods to Modulate Pre-mRNA Splicing -- Using Yeast Genetics to Study Splicing Mechanisms -- Medium Throughput Analysis of Alternative Splicing by Fluorescently Labeled RT-PCR -- Chromatin Immunoprecipitation Approaches to Determine Co-Transcriptional Nature of Splicing -- Computational Approaches to Mine Publicly Available Databases -- Approaches to Link RNA Secondary Structures with Splicing Regulation -- Methods to Study Splicing from High-Throughput RNA Sequencing Data -- Global Protein-RNA Interaction Mapping at Single Nucleotide Resolution by iCLIP-Seq -- Predicting Alternative Splicing.
Keywords: Human genetics; Human Genetics