Synthetic Biology - Polizzi, Karen M.; Kontoravdi, Cleo.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2013)
What is Synthetic Biology? -- Gene Synthesis Method Based on Overlap Extension PCR and DNAWorks Program -- BioBrick™ Assembly Using the In-Fusion PCR Cloning Kit -- Overlap Extension PCR Cloning -- One-Step Isothermal Assembly of DNA Fragments -- Creation and Characterization of Component Libraries for Synthetic Biology -- In vivo and in vitro Characterisation of s70 Constitutive Promoter BioParts by Real-time PCR and Fluorescent Measurements -- In Vivo Screening of Artificial Small RNAs for Silencing Endogenous Genes in Escherichia coli -- Construction and Engineering of  Large Biochemical Pathways via DNA Assembler -- Assembly of Multi-gene Pathways and Combinatorial Pathway Libraries through ePathBrick Vectors -- Tandem Recombineering by SLIC Cloning and Cre-LoxP Fusion to Generate Multigene Expression Constructs for Protein Complex Research -- Combinatorial DNA Assembly using Golden Gate Cloning -- Construction of Synthetic Gene Circuits in the Escherichia coli Genome -- Shuffling of DNA Cassettes in a Synthetic Integrin -- Systematic Methodology for the Development of Mathematical Models for Biological Processes -- SOBOLHDMR: A General Purpose Modelling Software.
Keywords: Biotechnology; Biochemical engineering; Biotechnology; Biochemical Engineering