Rolling Circle Amplification (RCA) : Toward New Clinical Diagnostics and Therapeutics - Demidov, Vadim V.; SpringerLink (Online service) (Cham : Springer International Publishing : Imprint: Springer, 2016)
Preface -- Part 1. Improved DNA polymerases and new DNA and DNA/RNA ligases useful in RCA -- 1. Improvement of φ29 DNA polymerase amplification performance by fusion of DNA binding motifs -- 2. Preparation of circular templates by T4 RNA ligase 2 for rolling circle amplification of target microRNAs with high specificity and sensitivity -- 3. Use of DNA CircLigase for direct isothermal detection of microbial mRNAs by RNA-primed rolling circle amplification and preparation of ø29 DNA polymerase not contaminated -- Part 2. RCA-involving techniques with enhanced signal amplification -- 4. Nicking enzyme-assisted branched-chain RCA reaction for cascade DNA amplification -- 5. Combining isothermal amplification techniques: Coupled RCA-LAMP -- Part 3. Emerging RCA diagnostics -- 6. Detection of vascular disease-related single nucleotide polymorphisms in clinical samples using ramified rolling circle amplification -- 7. Ultrasensitive isothermal detection of protein analytes using rolling circle amplification in micro-scale platforms -- 8. Rolling circle amplification with padlock probes for in situ detection of RNA analytes -- 9. PNA-assisted rolling circle amplification for detection of DNA marker sequences in human cells -- 10. Sensor systems with magnetic and optomagnetic readout of rolling circle amplification products -- Part 4. Prospective RCA-based therapeutics -- 11. DNA nanoclews for stimuli-responsive anticancer drug delivery -- 12. RCA-assisted self-assembled DNA origami nano-constructs as vehicles for cellular delivery of diagnostic probes and therapeutic drugs -- 13. DeNAno: a novel multivalent affinity reagent produced by selection of RCA-generated DNA nanoparticle libraries -- Index.
Keywords: Enzymology; Biochemical engineering; Medical biochemistry; Enzymology; Biochemical Engineering; Medical Biochemistry