Semaphorin Signaling : Methods and Protocols - Terman, Jonathan R.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
Semaphorins and their Signaling Mechanisms -- Isolating Fc-Tagged SEMA4D Recombinant Protein from 293FT Cells -- Expression and Purification of Class 7 Semaphorin and its Plexinc1 Receptor Using Baculovirus-Mediated Mammalian Cell Gene Transduction -- Immunoaffinity Purification of the Glycosylated Extracellular Fragment of Mouse Plexin A2 Produced in a Mammalian Expression Systems -- Plate-Based Assay for Measuring Direct Semaphorin-Neuropilin Interactions -- Characterizing Plexin Gtpase Interactions Using Gel Filtration, Surface Plasmon Resonance Spectrometry and Isothermal Titration Calorimetry -- In Vitro Assay for the Rap Gtpase Activating Protein Activity of the Purified Cytoplasmic Domain of Plexin -- Characterizing F-Actin Disassembly Induced by the Semaphorin Signaling Component -- Characterizing Erbb-2-Mediated Tyrosine Phosphorylation and Activation of Plexins -- Characterizing PKA-Mediated Phosphorylation of Plexin Using Purified Proteins -- Using Heterologous Cos-7 Cells to Identify Semaphorin Signaling Components -- Analysis of Semaphorin-Induced Growth Cone Collapse and Axon Growth Inhibition -- Using Rotary Shadow Electron Microscopy to Characterize Semaphorin-Mediated Growth Cone Collapse -- An Electrical Impedance-Based Method for Quantitative Real-Time Analysis of Semaphorin-Elicited Endothelial Cell Collapse -- Regulation of Cortical Dendrite Morphology and Spine Organization by Secreted Semaphorins - A Primary Culture Approach -- Characterizing Semaphorin Signaling Using Isolated Neurons in Culture -- Performing Axon Orientation Assays With Secreted Semaphorins And Other Guidance Cues -- Assays to Examine Transmembrane Semaphorin Function In Vitro -- Micro-CALI to Study Localized Roles of the Semaphorin Signaling Component CRMP in Axon Growth -- Visualizing and Characterizing Semaphorin Endocytic Events Using Quantum Dot Conjugated Proteins -- Visualization of Clathrin-Mediated Endocytosis during Semaphorin-Guided Axonal Growth -- Tracking Differential Endocytosis and Trafficking of Semaphorin Receptor Complexes in Responding Nerve Growth Cones -- Antibody-Feeding Assay: A Method to Track the Internalization of Neuropilin-1 and Other Cell Surface Receptors -- Photolithography-Based Substrate Microfabrication for Patterning Semaphorin 3A to Study Neuronal Development -- Characterization of Semaphorin 6A-Mediated Effects on Angiogenesis through Regulation of VEGF Signaling -- Studying the Effects of Semaphorins on Oligodendrocyte Lineage Cells -- Characterizing Semaphorin-Mediated Immune Responses Using an Antigen-Presentation Assay -- Podocyte Shape Regulation by Semaphorin 3A and MICAL-1 -- In Vivo and In Vitro Knockdown Approaches in the Avian Embryo as a Means to Study Semaphorin Signaling -- Assaying the Action of Secreted Semaphorins on Vascular Permeability -- Characterization of the Effects of Semaphorin 4D Signaling on Angiogenesis -- Characterizing Semaphorin-Mediated Effects on Sensory and Motor Axon Pathfinding and Connectivity during Embryonic Development -- Experimental Approaches for Studying Semaphorin Signals in Tumor Growth and Metastasis in Mouse Models -- Characterizing Semaphorin Signaling In Vivo Using C. Elegans. .
Keywords: Proteins .; Protein Science