Unnatural Amino Acids : Methods and Protocols - Pollegioni, Loredano; Servi, Stefano; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2012)
Preparation of Unnatural Amino Acids with Ammonia-lyases and 2,3-aminomutases -- Multistep Enzyme Catalyzed Reactions for Unnatural Amino Acids -- Enzymatic Production of Enantiopure Amino Acids from Mono-substituted Hydantoin Substrates -- Preparation of Glutamate Analogues by Enzymatic Transamination -- Carbon-Carbon Bond Forming Enzymes for the Synthesis of Non-natural Amino Acids.-  Engineering Cyclic Amidases for Non-natural Amino Acid Synthesis -- NMR Analysis of Unnatural Amino Acids in Natural Antibiotics -- Site-specific Incorporation of Unnatural Amino Acids as Probes for Protein Conformational Changes -- Application of Unnatural Amino Acids to the De novo Design of Selective Antibiotic Peptides -- Use of Unnatural Amino Acids to Probe Structure-activity Relationships and Mode-of-action of Antimicrobial Peptides -- Experimental Methods for Scanning Unnatural Amino Acid Mutagenesis -- Genetic Incorporation of Unnatural Amino Acids into Proteins in Yeast -- Site-specific Incorporation of Unnatural Amino Acids into Proteins in Mammalian Cells -- Incorporation of Unnatural Non-a-amino Acids into the N-terminus of Proteins in a Cell-free Translation System -- Site Specific Modification of Proteins by the Staudinger-phosphite Reaction -- HPLC Methods for Determination of D-aspartate and N-methyl-D-aspartate -- Estimation of Chronological Age from the Racemization Rate of L- and D-aspartic Acid: How to Completely Separate Enantiomers from Dentin -- Enzymatic Detection of D-amino Acids -- An Enzymatic-HPLC Assay to Monitor Endogenous D-serine Release from Neuronal Cultures -- Electrophysiological Analysis of the Modulation of NMDA-receptors Function by d-serine and Glycine in the Central Nervous System -- Biosensors for D-amino Acid Detection -- Analysis of D-β-aspartyl Isomers at Specific Sites in Proteins -- Nutritional Value of D-amino Acids, D-peptides and Amino Acid Derivatives in Mice -- Preparation and Assay of Recombinant Serine Racemase -- Assay of Amino Acid Racemases -- Assays of D-amino Acid Oxidases -- Enzymes Acting on D-amino Acid Containing Peptides.
Keywords: Biochemistry; Enzymology; Biochemistry, general; Enzymology