Serum/Plasma Proteomics : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Simpson, Richard J. (Editor), Greening, David W. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2011.
Edition:1st ed. 2011.
Series:Methods in Molecular Biology, 728
Subjects:
Table of Contents:
  • Fractionation Strategies for In-depth Blood Proteome Analysis. Plasma Biomarker Discovery using 3-D Protein Profiling Coupled with Label-free Quantitation. Intact Protein Separation by 1 and 2-dimension Liquid Chromatography for Comparative Proteomic Separation of Partitioned Serum or Plasma. In-depth Analysis of a Plasma or Serum Proteome using a 4-D Protein Profiling Method. Intact-protein Analysis System (IPAS) for Discovery of Serum-based Disease Biomarkers. Model-based Discovery of Circulating Biomarkers. Low-molecular Weight Plasma Proteome Analysis using Centrifugal Ultrafiltration. High-throughput Analysis of Glycoproteins from Plasma
  • Blood Collection and Handling Strategies. Minimizing Pre-analytical Variation of Plasma Samples by Proper Blood Collection and Handling. Collection and Handling of Blood Specimens for Peptidomics. Investigation of Peptide Biomarker Stability in Plasma Samples using Time-course MS Analysis
  • Methods for Quantitative Assaying of Blood-Based Biomarkers. Biomarker Validation in Blood Specimens by Selected Reaction Monitoring Mass Spectrometry of N-glycosites. A Fluorescent Microsphere-based Method for Assay of Multiple Analytes in Plasma. Immuno-mass Spectrometry: Quantification of Low Abundance Proteins in Biological Fluids. Qualitative and Quantitative Analysis of Glycated Proteins in Human Plasma by Glucose Isotopic Labeling with 13C6-reducing Sugars
  • Proteome Analysis of Blood Cell Components, Vesicles, and Blood-related Fluids. Exosome Isolation for Proteomic Analyses and RNA Profiling. Extraction and Proteome Analysis of Liver Tissue Interstitial Fluid. A Protocol for the Preparation of Cryoprecipitate and Cryo-depleted Plasma
  • Preparation of Platelet Concentrates. Phosphoproteome Analysis of the Platelet Plasma Membrane
  • Bioinformatic Analysis of Blood Proteins and Peptides. Statistical Design and Analysis of Label-free LC-MS Proteomic Experiments: A Case Study of Coronary Artery Disease. Data Management in Mass Spectrometry-based Proteomics. Bioinformatics Challenges in the Proteomic Analysis of Human Plasma. Using the Human Plasma PeptideAtlas to Study Human Plasma Proteins.