Ribozyme Protocols - Turner, Philip C.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1997)
Ribozymes -- Computer-Aided Calculation of the Local Folding Potential of Target RNA and Its Use for Ribozyme Design -- Computational Approaches to the Identification of Ribozyme Target Sites -- Computer Analysis of the Conservation and Uniqueness of Ribozyme-Targeted HIV Sequences -- Selection of Accessible Sites for Ribozymes on Large RNA Transcripts -- Selection of Efficient Ribozyme Cleavage Sites in Target RNAs -- Chemical Synthesis, Analysis, and Purification of Ribozymes -- A Practical Method for the Production of RNA and Ribozymes -- Preparation of Templates for Production of Ribozymes and Substrates -- Enzymatic Synthesis and Characterization of Unmodified Ribozymes and Substrates -- T7 Transcript Length Determination Using Enzymatic RNA Sequencing -- Chemical and Enzymatic Approaches to Construct Modified RNAs -- Applications of Modified Transcripts -- Cloning Strategies for Catalytic Antisense RNAs -- PCR-Based Construction of Long Hammerhead Ribozymes -- Design and Production of Asymmetric Hammerhead Ribozymes -- Minimized Hammerhead Ribozymes -- Design of Hairpin Ribozymes for In Vitro and Cellular Applications -- Design of the Hairpin Ribozyme for Targeting Specific RNA Sequences -- Design and Preparation of Sequence-Specific RNase P Ribozymes -- Theoretical Considerations in Measuring Reaction Parameters -- Experimental Approaches for Measuring Reaction Parameters -- Determination of Catalytic Parameters for Hairpin Ribozymes -- Characterizing Ribozyme Cleavage Reactions -- Defining Optimum Reaction Conditions for Hammerhead Ribozymes -- Using Fluorescence Resonance Energy Transfer to Investigate Hammerhead Ribozyme Kinetics -- Design of Hybridizing Arms in Hammerhead Ribozymes -- Optimization of Hammerhead Flanking Sequences Using Oligonucleotide Facilitators -- Enhancement of Ribozyme Function by RNA Binding Proteins -- Selection of Fast-Hybridizing Complementary RNA Species In Vitro -- In Vitro Selection of Hairpin Ribozymes -- The Detection of Hammerhead Ribozyme Cleavage by RT-PCR Methods -- Detection of Ribozyme Cleavage Products Using Reverse Ligation-Mediated PCR (RL-PCR) -- Quantitation of Ribozyme Target Abundance by QCPCR -- Trans-Splicing Reactions by Ribozymes -- Ligation of RNA Molecules by the Hairpin Ribozyme -- Mutagenesis and Modeling of the Hairpin Ribozyme Family -- Preparation of Homogeneous Ribozyme RNA for Crystallization -- Establishing Suitability of RNA Preparations for Crystallization -- A Sparse Matrix Approach to Crystallizing Ribozymes and RNA Motifs -- Crystallographic Analyses of Chemically Synthesized Modified Hammerhead RNA Sequences as a General Approach Toward Understanding Ribozyme Structure and Function -- tRNA Delivery Systems for Ribozymes -- Expressing Ribozymes in Plants -- Using Microinjection of Xenopus Oocytes to Express and Optimize Ribozymes In Vivo -- Exogenous Cellular Delivery of Ribozymes and Ribozyme Encoding DNAs -- Optimization of Lipid-Mediated Ribozyme Delivery to Cells in Culture -- Retroviral Delivery and Anti-HIV Testing of Hammerhead Ribozymes -- Hairpin Ribozyme Gene Therapy for AIDS -- Clinical Aspects of Ribozymes as Therapeutics in Gene Therapy.
Keywords: Biochemistry; Biochemistry, general