Schizosaccharomyces pombe : Methods and Protocols - Singleton, Teresa L.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2018)
Preparation of Solutions and Reagents -- Analysis of Fission Yeast Single DNA Molecules on the Megabase Scale using DNA Combing -- Chromatin Immunoprecipitation - Polymerase Chain Reaction (ChIP-PCR) Detects Methylation, Acetylation, and Ubiquitylation in S. pombe -- Primer Design and Inverse PCR on Yeast Display Antibody Selection Outputs -- Molecular Cloning and Characterization of Small Viral Genome in Fission Yeast -- Total RNA Isolation and Quantification of Specific RNAs in Fission Yeast -- Analysis of Reverse Transcribed mRNA using PCR and Polyacrylamide Gel Electrophoresis -- The No-nonsens SDS-PAGE -- Crystallization of Recombinant α-actinin and Related Proteins -- Estimation of GFP-nucleoporin Amount Based on Fluorescence Microscopy -- Antibody Pull-down Experiments in Fission Yeast -- Preparation of Cell Lysates of Fission Yeast for Immunoprecipitation -- Wide-band Electrical Impedance Spectroscopy (EIS) Measures S. pombe Cell Growth in vivo -- In Situ Chromatin Binding Assay Using Epifluorescent Microscopy in S. pombe -- High-frequency Lithium Acetate Transformation of Schizosaccharomyces pomTetrad Dissection in Fission Yeast -- Random Spore Analysis in Fission Yeast -- Duplication and Transformation of the Schizosaccharomyces pombe Collection of Deletion Strains -- Schizosaccharomyces pombe Biotechnological Applications in Winemaking -- Schizosaccharomyces pombe Isolation Protocol. .
Keywords: Microbiology; Microbiology