Transcriptional Regulation : Methods and Protocols /
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2012.
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| Edition: | 1st ed. 2012. |
| Series: | Methods in Molecular Biology,
809 |
| Subjects: |
Table of Contents:
- Genome Wide In Vivo Crosslinking of Sequence Specific Transcription Factors
- Characterization of Complex Regulatory Networks and Identification of Promoter Regulatory Elements in Yeast: "In Silico" and "Wet-Lab" Approaches
- Electrophoretic Mobility Shift Assay Analysis of NFkB Transcriptional Regulation by Nuclear IkBa
- Probing Endogenous RNA Polymerase II Pre-Initiation Complexes by Electrophoretic Mobility Shift Assay
- Elucidating Protein: DNA Complex by Oligonucleotide DNA Affinity Purification
- Chromatin Immunoprecipitation (ChIP) Assay as Tool for Analyzing Transcription Factor Activity
- Two-Step Crosslinking for Analysis of Protein-Chromatin Interactions
- Chromatin Immunoprecipitation Analysis of NFkB Transcriptional Regulation by Nuclear IkBa in Human Macrophages
- In Vivo ChIP for the Analysis of Microdissected Tissue Samples
- Quantification of Protein-DNA Interactions by In Vivo Chromatin Immunoprecipitation in Yeast
- Mapping Protein-DNA Interactions Using ChIP-Sequencing
- ChIP and ReChIP Assays: Investigating Interactions Between Regulatory Proteins, Histone Modifications, and the DNA Sequences to which They Bind
- Transcriptional Regulation of Genes Via Hypoxia-Inducible Factor
- Exchange Protein Directly Activated By Cyclic AMP 1 (Epac1)-Regulated Recruitment of CCAAT/Enhancer Binding Proteins (C/EBPs) to the Suppressor of Cytokine Signalling-3 (SOCS-3) Promoter
- Computational Analysis of Promoter Elements and Chromatin Features in Yeast
- Chromatin Affinity Purification (ChAP)
- Determination of Histone Acetylation Status by Chromatin Immunoprecipitation
- Immunostaining of Drosophila Polytene Chromosomes to Investigate Recruitment of Chromatin Binding Proteins
- Detection of Transcriptional Activators, Co-Activators, and Chromatin Remodeling by Chromatin Immunoprecipitation Coupled with Real-Time PCR
- Chromatin Endogenous Cleavage and Psoralen Crosslinking Assays to Analyze rRNA Gene Chromatin In Vivo
- UV-Induced DNA Damage and DNA Repair in Ribosomal Genes Chromatin
- Analysis of SUC2 Promoter Structure by Nucleosome Scanning
- Chromatin Immunoprecipitation of Mouse Embryos
- Chromatin Immunoprecipitation (ChIP) in Mouse Hippocampal Cells and Tissues
- Approaches for Studying Nucleosome Movement by ATP-Dependent Chromatin Remodeling Complexes
- Mapping Protein-DNA and Protein-Protein Interactions of ATP-Dependent Chromatin Remodelers
- Evaluation of Histone Modifying Enzymes in Stem Cell Populations
- Purification of Multiprotein Histone Acetyltransferase Complexes
- Reconstitution of Active and Stoichiometric Multisubunit Lysine Acetyltransferase Complexes in Insect Cells
- Affinity Purification of MLL3/MLL4 Histone H3K4 Methyltransferase Complex
- Methods for Analyzing Histone Citrullination in Chromatin Structure and Gene Regulation
- Analysis of mRNA Abundance and Stability by Ribonuclease Protection Assay
- Array-Based Nuclear Run-On (ANRO) Analysis
- In Vivo Run on Assays to Monitor Nascent Precursor RNA Transcripts
- Genome Wide Full-Length Transcript Analysis Using 5' and 3' Paired-End-Tag Next Generation Sequencing (RNA-PET)
- Analysis of Cotranscriptionnal RNA Processing by RNA-ChIP Assay
- Quantitative Analysis of Transcription Elongation by RNA Polymerase I In Vitro
- Detection and Characterization of Transcription Termination
- Promoter-Associated Noncoding RNA from the CCND1 Promoter.