Transgenic Mouse Methods and Protocols - Hofker, Marten H.; van Deursen, Jan.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2011)
Introduction: Strategies for developing genetically modified mice -- Genetic Modification of the Mouse; General Technology: Pronuclear and Blastocyst Injection -- Generation of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos -- Cryopreservation of mouse spermatozoa and In vitro fertilization -- Autopsy and histological analysis of the transgenic mouse -- Transgene design -- Inducible Transgenic Mouse Models -- Lentiviral transgenesis -- Transgenesis in Mouse Embryonic Stem Cells -- Engineering BAC Reporter Gene Constructs for Mouse Transgenesis -- Targeting Vector Construction Through Recombineering -- Generating Conditional Knockout Mice -- Hypomorphic mice -- MICER Targeting Vectors for Manipulating the Mouse Genome -- Knockin approaches -- Generation of a series of knockin alleles using RMCE in ES cells -- Selection of targeted mutants from a library of randomly mutagenized ES cells -- Generation of genetically modified rodents using random ENU mutagenesis -- Bone marrow transplantations to study gene function in hematopoietic cells -- Adenovirus Mediated Gene Transfer.
Keywords: Animal genetics; Genetic engineering; Medical genetics; Animal Genetics and Genomics; Genetic Engineering; Gene Function