Single Molecule Enzymology : Methods and Protocols - Mashanov, Gregory I.; Batters, Christopher; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2011)
Single Enzyme Studies: A Historical Perspective -- Functional Surface-Attachment in a Sandwich Geometry of GFP-Labeled Motor Proteins -- Studying Kinesin's Enzymatic Cycle Using a Single-Motor Confocal Motility Assay, Employing Förster Resonance Energy Transfer -- Fluorescence Imaging with One Nanometer Accuracy: In Vitro and In Vivo Studies of Molecular Motors -- Snapshots of Kinesin Motors on Microtubule Tracks -- Structural and Dynamic Characterization of Biochemical Processes by Atomic Force Microscopy -- Using Optical Tweezers to Study Fine Details of Myosin ATPase Mechanochemical Cycle -- Quantum Dot Labeling Strategies to Characterize Single Molecular Motors -- Imaging Individual Myosin Molecules Within Living Cells -- Single Molecule Measurements Using Microneedles -- Fluorescent Nucleoside Triphosphates for Single Molecule Enzymology -- Probing the Mechanics of the Complete DNA Transcription Cycle in Real-Time Using Optical Tweezers -- A Single-Molecule Approach to Visualize the Unwinding Activity of DNA Helicases -- Real-Time Single-Molecule Observation of Green Fluorescent Protein Synthesis by Immobilized Ribosomes -- Single-Molecule Measurements of Topoisomerase Activity with Magnetic Tweezers -- Assembly of Recombinant Nucleosomes on Nanofabricated DNA Curtains for Single Molecule Imaging -- Simultaneous Observation of Chemomechanical Coupling of a Molecular Motor -- Microsecond Resolution of Single Molecule Rotation Catalyzed by Molecular Motors.
Keywords: Biochemistry; Enzymology; Biochemistry, general; Enzymology