Zinc Finger Proteins : Methods and Protocols - Liu, Jia.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2018)
Part 1. Basic Biology and Engineering of ZFPs -- PRDM14, a zinc finger protein, regulates cancer stemness -- Computational methods for analysis of the DNA-binding preferences of Cys2His2 zinc finger proteins -- Design and application of mA-specific Zinc finger proteins for the readout of DNA methylation -- Enhanced manipulation of human mitochondrial DNA heteroplasmy in vitro using tunable mtZFN technology -- Engineering RNA-binding proteins by modular assembly of RanBP2-type Zinc Fingers -- Design of a system for monitoring ubiquitination activities of E2 enzymes using engineered RING finger proteins -- Directed evolution of targeted recombinases for genome engineering -- Optical recording of cellular zinc dynamics with zinc finger based biosensors -- Delivery of superoxide dismutase using Cys2-His2 zinc-finger proteins -- Genome editing of MSCs as a platform for cell therapy -- Part 2. Methods for the Evaluation and Prevention of ZFN Cytotoxicity -- Integrated multimodal evaluation of genotoxicity in ZFN-modified primary human cells -- Selection and characterization of DNA aptamers against FokI nuclease domain -- An improved genome engineering method using surrogate reporter-coupled suicidal ZFNs -- Part 3. Delivery Methods of ZFPs -- Non-transgenic approach to deliver ZFNs in seeds for targeted genome engineering -- Gene editing in channel catfish via double electroporation of zinc finger nucleases -- Delivery of mtZFNs into early mouse embryos -- Stereotaxic surgery and viral-delivery of zinc finger epigenetic editing tools in rodent brain -- In-vivo applications of cell-penetrating zinc finger transcription factors -- Manufacturing and delivering genome-editing proteins.
Keywords: Human genetics; Human Genetics