Viral Vectors for Gene Therapy : Methods and Protocols - Manfredsson, Fredric P.; Benskey, Matthew J.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2019)
Basic Concepts in Viral Vector-Mediated Gene Therapy -- Design and Assembly of CRISPR/Cas9 Lentiviral and rAAV Vectors for Targeted Genome Editing -- Design, Construction, and Application of Transcription Activation-Like Effectors -- Practical Considerations for the use of DREADD and Other Chemogenetic Receptors to Regulate Neuronal Activity in the Mammalian Brain -- AAV Production using Baculovirus Expression Vector System -- Multimodal Production of Adeno-Associated Virus -- Generation of High Titer Pseudotyped Lentiviral Particles -- A Scalable Lentiviral Vector Production and Purification Method using Mustang Q Chromatography and Tangential Flow Filtration -- Current Use of Adenovirus Vectors and their Purification Methods -- Construction of Oncolytic Herpes Simplex Virus with Therapeutic Genes of Interest -- Poxviruses as Gene Therapy Vectors: Generating Poxviral Vectors Expressing Therapeutic Transgenes -- AAV Mediated Gene Delivery to the Mouse Liver -- Surgical Methods for Inner Ear Gene Delivery in Neonatal Mouse -- Gene Transfer to Mouse Kidney In Vivo -- Co-Delivery of a Short-Hairpin RNA and a shRNA-Resistant Replacement Gene with Adeno-Associated Virus: An Allele-Independent Strategy for Autosomal Dominant Retinal Disorders -- Localized Intra-Arterial Gene Delivery using AAV -- Stable Genetic Modification of Mesenchymal Stromal Cells Using Lentiviral Vectors -- Systemic Delivery of Adeno-Associated Viral Vectors in Mice and Dogs -- Intrathecal Delivery of AAV Vectors in Cynomolgus Macaques for CNS Gene Therapy and Gene Expression Analysis in Microdissected Motor Neurons -- Detailed Method for Intrathecal Delivery of Gene Therapeutics by Direct Lumbar Puncture in Mice -- Cerebromedullary Cistern Injection of Viral Vectors in Non-Human Primates.
Keywords: Gene therapy; Virology; Gene Therapy; Virology