Vertebrate Embryogenesis : Embryological, Cellular, and Genetic Methods - Pelegri, Francisco J.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2019)
Culture and Host Transfer of Xenopus Oocytes for Maternal mRNA Depletion and Genome Editing Experiments -- Immunofluorescence of Microtubule Assemblies in Amphibian Oocytes and Early Embryos -- Assaying NanoLuc Luciferase Activity from mRNA-Injected Xenopus Embryos -- Reconstitution of Intracellular Calcium Signaling in Xenopus Egg Extracts -- Membrane Microdomains as Platform to Study Membrane-Associated Events during Oogenesis, Meiotic Maturation, and Fertilization in Xenopus laevis -- Embryological and Genetic Manipulation of Chick Development -- Identifying Protein-DNA and Protein-Protein Interactions in Avian Embryos -- Experimental Manipulation of Ploidy in Zebrafish Embryos and its Application in Genetic Screens -- RNA Tomography for Spatially Resolved Transcriptomics (Tomo-Seq) -- Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos -- Ex Utero Culture and Imaging of Mouse Embryos -- Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections -- Reptile Embryology and Regeneration -- Manipulation of Developmental Function in Turtles with Notes on Alligators -- Methods for Isolating the Balbiani Body/Germ Plasm from Xenopus laevis Oocytes -- Visualizing the Balbiani Body in Zebrafish Oocytes -- Isolation of Zebrafish Balbiani Bodies for Proteomic Analysis -- Generation of Xenopus Haploid, Triploid, and Hybrid Embryos -- Germ Cell Transplantation in Avian Species -- Production of Germ-Line Chimeras in Zebrafish -- A Method for Zebrafish Follicle Transplantation into Recipient Mothers for the Generation of Fertilizable Eggs and Viable Offspring -- Optimized Protocol of Zebrafish Somatic Cell Nuclear Transfer (SCNT) -- An Accessible Protocol for the Generation of CRISPR- Cas9 Knockouts Using INDELs in Zebrafish -- Modeling Embryonic Cleavage Patterns -- Laboratory Guidelines for Animal Care.
Keywords: Embryology; Animal models in research; Animal genetics; Embryology; Animal Models; Animal Genetics and Genomics