Viral Vectors for Gene Therapy : Methods and Protocols - Merten, Otto-Wilhelm.; Al-Rubeai, Mohamed; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2011)
Introduction to Viral Vectors -- Introduction to Gene Therapy: A Clinical Aftermath -- Host Cells and Cell Banking -- Overview of Current Scalable Methods for Purification of Viral Vectors -- Methods to Construct Recombinant Adenovirus Vectors -- Manufacturing of Adenoviruses Vectors: Production and Purification of Helper Dependent Adenovirus -- Manufacturing of Retroviruses -- Lentiviral Vectors -- Adeno-Associated Viruses -- Manufacturing of Adeno-Associated Viruses, for Example: AAV2 -- Vector Characterization Methods for Quality Control Testing of Recombinant Adeno-Associated Viruses -- Baculoviruses Mediate Efficient Gene Expression in a Wide Range of Vertebrate Cells -- Herpes Simplex Virus Type 1 (HSV-1)-Derived Recombinant and Amplicon Vectors -- Manufacture of Measles Viruses -- In Vivo Gene Delivery into hCD34+ Cells in a Humanized Mouse Model -- In Vivo Evaluation of Gene Transfer into Mesenchymal Cells (in View of Cartilage Repair) -- Ethical Consideration -- Clinical Trials of GMP Products in the Gene Therapy Field.
Keywords: Human genetics; Virology; Gene therapy; Human Genetics; Virology; Gene Therapy