miRNA Maturation : Methods and Protocols - Arenz, Christoph; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2014)
The Pathway of miRNA Maturation -- Micro RNA Maturation and Human Disease -- Approaches to the Modulation of miRNA Maturation -- Expression Profiling of Components of the miRNA Maturation Machinery -- Primary microRNA Processing Assay Reconstituted using Recombinant Drosha and DGCR8 -- In-vivo processing Assay Based on a Dual Luciferase Reporter System to Evaluate DROSHA Enzymatic Activity -- Assaying Dicer-mediated miRNA Maturation by Means of Fluorescent Substrates -- A Fluorescence Correlation Spectroscopy-based Enzyme Assay for Human Dicer -- Detection of Micro RNA Maturation Using Unmodified pre-miRNA and Branched Rolling Circle Amplification -- Identification of Inhibitors of microRNA Function from Small Molecule Screens -- Quantitative RT-PCR Specific for Precursor and Mature miRNAs -- Cellular MicroRNA Sensors Based on Luciferase Reporters -- Inhibition of miRNA maturation by Peptide Nucleic Acids -- Molecular Methods for Validation of the Biological Activity of Peptide Nucleic Acids Targeting MicroRNAs -- Lentiviral Overexpression of miRNAs.
Keywords: Human genetics; Gene expression; Human Genetics; Gene Expression