Zygotic Genome Activation : Methods and Protocols - Lee, Kiho.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
Clearance of Maternal RNAs: Not a Mummy's Embryo Anymore -- Link of Zygotic Genome Activation and Cell Cycle Control -- Role of MicroRNAs in Zygotic Genome Activation: Modulation of mRNA during Embryogenesis -- Gene Expression Analysis of Mammalian Oocytes and Embryos by Quantitative Real-Time RT-PCR -- Detection of miRNA in Mammalian Oocytes and Embryos -- Detection of Bidirectional Promoter-Derived IncRNAs from Small-Scale Samples using Pre-Amplification-Free Directional RNA-Seq Method -- Detection and Characterization of Small Non-Coding RNAs in Mouse Gametes and Embryos Prior to Zygotic Genome Activation -- Purification of Zygotically Transcribed RNA through Metabolic Labeling of Early Zebrafish Embryos -- RNA FISH to Study Zygotic Genome Activation in Early Mouse Embryos -- Detection of RNA Polymerase II in Mice Embryos during Zygotic Genome Activation using Immunocytochemistry -- Immunological Staining of Global Changes in DNA Methylation in the Early Mammalian Embryo -- Single Cell Restriction Enzyme-Based Analysis of Methylation at Genomic Imprinted Regions in Preimplantation Mouse Embryos -- Use of Chemicals to Inhibit DNA Replication, Transcription, and Protein Synthesis to Study Zygotic Genome Activation -- Targeted Gene Knockdown in Early Embryos using siRNA -- Generating Mouse Models using Zygote Electroporation of Nucleases (ZEN) Technology with High Efficiency and Throughput -- CRISPR/Cas9-Mediated Gene Targeting during Embryogenesis in Swine -- Potential Involvement of SCF-Complex in Zygotic Genome Activation during Early Bovine Embryo Development -- Use of Histone K-M Mutants for the Analysis of Transcriptional Regulation in Mouse Zygotes.
Keywords: Human genetics; Human Genetics